Detailed information of TA system TA021689

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 152611..153204
• Replicon: chromosome
• Accession: NC_008344
• Organism: Nitrosomonas eutropha C91

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: NEUT_RS00775
• Protein ID: WP_049753074.1
• Coordinates: 152926..153204 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: NEUT_RS00770
• Protein ID: WP_011633283.1
• Coordinates: 152611..152916 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NEUT_RS00745	148745..149563	+	819	WP_011633279.1	ABC transporter permease	-
NEUT_RS00750	149712..149915	+	204	WP_049753073.1	hypothetical protein	-
NEUT_RS00755	150090..150644	+	555	WP_011633281.1	ORF6N domain-containing protein	-
NEUT_RS00765	151086..152135	+	1050	WP_011633282.1	Rpn family recombination-promoting nuclease/putative transposase	-
NEUT_RS00770	152611..152916	-	306	WP_011633283.1	HigA family addiction module antidote protein	Antitoxin
NEUT_RS00775	152926..153204	-	279	WP_049753074.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
NEUT_RS00780	153294..154607	+	1314	WP_011633285.1	ABC transporter ATP-binding protein	-
NEUT_RS13455	154747..158178	+	3432	WP_166484980.1	glycosyltransferase family 4 protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (25-66)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10529.88 Da
• Isoelectric Point: 5.8184

>T21689 WP_049753074.1 NC_008344:c153204-152926 [Nitrosomonas eutropha C91]
MIQSFRCKSTQAVFEGEYPQRFSAIQAAAERKLAQLEAAQTLDFLRSPPGNRLEKLAGDREGQWSIRINAQWRICFTWSD
LGPADVEIVDYH

Nucleotide

• Length: 279 bp

>T21689 NC_008344:c153204-152926 [Nitrosomonas eutropha C91]
ATGATCCAGTCTTTTCGATGTAAGAGTACGCAAGCAGTGTTTGAGGGTGAATATCCTCAGCGTTTTAGTGCTATCCAAGC
TGCAGCTGAACGTAAGCTGGCACAATTGGAGGCAGCACAAACACTTGATTTTTTGCGTTCTCCACCAGGAAATCGGCTGG
AAAAACTGGCTGGCGACCGGGAAGGTCAATGGAGCATTCGTATCAACGCACAATGGCGTATATGCTTTACTTGGTCTGAT
CTTGGTCCTGCCGATGTAGAGATTGTTGATTATCATTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11402.05 Da
• Isoelectric Point: 8.0919

>AT21689 WP_011633283.1 NC_008344:c152916-152611 [Nitrosomonas eutropha C91]
MTRPVNCMRAVHPGEVLREDFLIPAGISVNALAIALSVPATRIHEIVKERRAVTADTAERLAHYFGGDAASWLALQASYD
LKTLPTRDEIERRVQRREEHR

Nucleotide

• Length: 306 bp

>AT21689 NC_008344:c152916-152611 [Nitrosomonas eutropha C91]
ATGACTAGACCTGTAAACTGCATGCGTGCCGTCCATCCCGGTGAGGTACTGCGTGAAGATTTTTTGATTCCAGCTGGAAT
AAGTGTCAACGCTCTGGCGATTGCCTTGAGTGTGCCAGCCACCCGCATTCATGAAATCGTCAAGGAGCGGCGCGCCGTTA
CCGCTGATACTGCTGAACGGCTGGCGCATTATTTCGGGGGTGATGCTGCTTCCTGGCTTGCTTTGCAGGCCAGCTACGAT
CTGAAAACACTGCCCACGCGCGATGAAATTGAACGCCGCGTGCAAAGACGTGAGGAACACCGGTGA