Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 88744..89346 Replicon chromosome
Accession NZ_CP083620
Organism Enterobacter bugandensis strain FDAARGOS_1520

Toxin (Protein)


Gene name higB Uniprot ID A0A167J774
Locus tag LA316_RS00405 Protein ID WP_047366784.1
Coordinates 88744..89055 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A167J796
Locus tag LA316_RS00410 Protein ID WP_047366785.1
Coordinates 89056..89346 (+) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LA316_RS00375 (LA316_00375) 84403..85146 + 744 WP_048959977.1 winged helix-turn-helix domain-containing protein -
LA316_RS00380 (LA316_00380) 85143..85613 + 471 WP_028014878.1 hypothetical protein -
LA316_RS00385 (LA316_00385) 85701..86300 + 600 WP_045260234.1 glucose-1-phosphatase -
LA316_RS00390 (LA316_00390) 86294..87175 + 882 WP_045260235.1 virulence factor BrkB family protein -
LA316_RS00395 (LA316_00395) 87172..87609 + 438 WP_008501836.1 D-aminoacyl-tRNA deacylase -
LA316_RS00400 (LA316_00400) 87654..88595 + 942 WP_047366783.1 fatty acid biosynthesis protein FabY -
LA316_RS00405 (LA316_00405) 88744..89055 + 312 WP_047366784.1 hypothetical protein Toxin
LA316_RS00410 (LA316_00410) 89056..89346 + 291 WP_047366785.1 NadS family protein Antitoxin
LA316_RS00415 (LA316_00415) 89388..90317 - 930 WP_063437456.1 formate dehydrogenase accessory protein FdhE -
LA316_RS00420 (LA316_00420) 90314..90949 - 636 WP_015569914.1 formate dehydrogenase cytochrome b556 subunit -
LA316_RS00425 (LA316_00425) 90946..91848 - 903 WP_003861960.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12135.13 Da        Isoelectric Point: 8.8415

>T216888 WP_047366784.1 NZ_CP083620:88744-89055 [Enterobacter bugandensis]
MLFIETDIFTEDVKTLLDDDEYHRFQIFLATQPEYGDVIQNTGGLRKIRWLAGGKGKRGGVRVIYFYRTCEFEIRLLLIY
RKGIKDDLSAGEKAILKKMIERW

Download         Length: 312 bp

>T216888 NZ_CP113503:4641046-4641420 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10674.22 Da        Isoelectric Point: 10.5888

>AT216888 WP_047366785.1 NZ_CP083620:89056-89346 [Enterobacter bugandensis]
MDKALFERLTQSVGQMNEIAEGQREPSRIFQIDAMKIKEIRQASGLSQSKFATLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVIPALSQ

Download         Length: 291 bp

>AT216888 NZ_CP113503:4640588-4640956 [Escherichia coli]
GTGTCAGACGCACTCTCCGGGACAATGCTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A167J774

Antitoxin

Source ID Structure
AlphaFold DB A0A167J796

References