Detailed information of TA system TA216875

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3197486..3198146
• Replicon: chromosome
• Accession: NZ_CP083613
• Organism: Enterobacter hormaechei subsp. oharae strain FDAARGOS_1533

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: LA353_RS15445
• Protein ID: WP_047053529.1
• Coordinates: 3197793..3198146 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A2J0Q4Z7
• Locus tag: LA353_RS15440
• Protein ID: WP_017383313.1
• Coordinates: 3197486..3197788 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LA353_RS15425 (LA353_15420)	3193656..3194981	+	1326	WP_047053533.1	SidA/IucD/PvdA family monooxygenase	-
LA353_RS15430 (LA353_15425)	3195008..3197197	+	2190	WP_047053531.1	TonB-dependent siderophore receptor	-
LA353_RS15440 (LA353_15435)	3197486..3197788	-	303	WP_017383313.1	XRE family transcriptional regulator	Antitoxin
LA353_RS15445 (LA353_15440)	3197793..3198146	-	354	WP_047053529.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LA353_RS15450 (LA353_15445)	3198337..3199287	-	951	WP_003859532.1	HTH-type transcriptional regulator Cbl	-
LA353_RS15455 (LA353_15450)	3199384..3200301	-	918	WP_003859531.1	nitrogen assimilation transcriptional regulator NAC	-
LA353_RS15465 (LA353_15460)	3200790..3201713	-	924	WP_047053526.1	L,D-transpeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (18-89)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13522.40 Da
• Isoelectric Point: 10.1729

>T216875 WP_047053529.1 NZ_CP083613:c3198146-3197793 [Enterobacter hormaechei subsp. oharae]
VWAIKTTDRFDDWFTSLNDSERASVLAALLVLREKGPGLSRPYADTLKGSRHSNMKELRIQSKGDPLRAFFAFNPNRTGI
VLCAGNKIGNERRFYDEMLLVADREYTRWLNTLKERN

Nucleotide

• Length: 354 bp

>T216875 NZ_CP113503:c1979613-1979239 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11028.85 Da
• Isoelectric Point: 6.4727

>AT216875 WP_017383313.1 NZ_CP083613:c3197788-3197486 [Enterobacter hormaechei subsp. oharae]
MGRTLEQLIADEKPEVVASAQAMATDILLNIHLAELREKVQKTQVDMARALGIKQPTVAVMEKAGRDIKLSSLKRYVEAA
GGKLRLDVELPDGSHYEFVL

Nucleotide

• Length: 303 bp

>AT216875 NZ_CP113503:c1980070-1979702 [Escherichia coli]
GTGTCAGACACACTGCCCGGGACAACGCATCCCGACGACAATCACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGAAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGATGCAGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAACGTAA

Structures

Antitoxin

• AlphaFold DB: A0A2J0Q4Z7