Detailed information of TA system TA216858

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 369847..370493
• Replicon: chromosome
• Accession: NZ_CP083613
• Organism: Enterobacter hormaechei subsp. oharae strain FDAARGOS_1533

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: LA353_RS01670
• Protein ID: WP_047050720.1
• Coordinates: 369847..370197 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A7H8UAV4
• Locus tag: LA353_RS01675
• Protein ID: WP_045335986.1
• Coordinates: 370194..370493 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LA353_RS01635 (LA353_01635)	365524..365994	+	471	WP_015703840.1	DNA repair protein RadC	-
LA353_RS01640 (LA353_01640)	366007..366333	+	327	WP_059443453.1	type IV toxin-antitoxin system YeeU family antitoxin	-
LA353_RS01645 (LA353_01645)	366354..366674	+	321	WP_059443702.1	TA system toxin CbtA family protein	-
LA353_RS01650 (LA353_01650)	366969..368525	+	1557	WP_047050722.1	hypothetical protein	-
LA353_RS01655 (LA353_01655)	368565..368741	-	177	WP_071962261.1	toxin-antitoxin system HicB family antitoxin	-
LA353_RS01660 (LA353_01660)	368878..369219	+	342	WP_006812446.1	SymE family type I addiction module toxin	-
LA353_RS01665 (LA353_01665)	369322..369692	+	371	Protein_315	hydroxyacid dehydrogenase	-
LA353_RS01670 (LA353_01670)	369847..370197	+	351	WP_047050720.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LA353_RS01675 (LA353_01675)	370194..370493	+	300	WP_045335986.1	XRE family transcriptional regulator	Antitoxin
LA353_RS01680 (LA353_01680)	370558..371439	-	882	WP_047050719.1	helix-turn-helix domain-containing protein	-
LA353_RS01685 (LA353_01685)	371682..372161	-	480	WP_047050718.1	hypothetical protein	-
LA353_RS01690 (LA353_01690)	372163..372951	-	789	WP_045329485.1	winged helix-turn-helix domain-containing protein	-
LA353_RS01695 (LA353_01695)	373785..374387	+	603	WP_047050717.1	LuxR C-terminal-related transcriptional regulator	-
LA353_RS01700 (LA353_01700)	374440..375024	+	585	WP_023299002.1	fimbrial protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	363303..384860	21557

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-88)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13497.59 Da
• Isoelectric Point: 8.8416

>T216858 WP_047050720.1 NZ_CP083613:369847-370197 [Enterobacter hormaechei subsp. oharae]
MWTVLFSKVFEQWLLEQEDGLQEKVLADLLNLQNYGPRLPRPYADTVKGSQYKNMKELRIQYAGRPVRAFFAFDPVRQAI
VLCAGDKRNDKTFYEKLIRIADAEFTLHLTSLEAAK

Nucleotide

• Length: 351 bp

>T216858 NZ_CP113493:c5014303-5013992 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTCACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11129.87 Da
• Isoelectric Point: 6.4714

>AT216858 WP_045335986.1 NZ_CP083613:370194-370493 [Enterobacter hormaechei subsp. oharae]
VKTLNEVIASRSPESQQRIQEMAEEMFLETGLQLLREELQLSQKHVADLMGVSQPAVTQLEQRGNDLKIATLKRYIEAMG
GKLSINVELPTGKRVAFHL

Nucleotide

• Length: 300 bp

>AT216858 NZ_CP113493:c5013991-5013701 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACACCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGAAC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACTGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAGGCACTTAACCGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A7H8UAV4