Detailed information of TA system TA216855

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): HipBST/HipA(toxin)
• Location: 9831..11182
• Replicon: chromosome
• Accession: NZ_CP083612
• Organism: Aeromonas enteropelogenes strain FDAARGOS_1536

Toxin (Protein)

• Gene name: HipT
• Uniprot ID: -
• Locus tag: LA358_RS00065
• Protein ID: WP_224210505.1
• Coordinates: 9831..10817 (-)
• Length: 329 a.a.

Antitoxin (Protein)

• Gene name: HipS
• Uniprot ID: -
• Locus tag: LA358_RS00070
• Protein ID: WP_124243609.1
• Coordinates: 10868..11182 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LA358_RS00035 (LA358_00035)	5597..6289	+	693	WP_224210502.1	B3/4 domain-containing protein	-
LA358_RS00040 (LA358_00040)	6369..7541	-	1173	WP_224210503.1	hypothetical protein	-
LA358_RS00045 (LA358_00045)	7943..8263	-	321	WP_042070761.1	hypothetical protein	-
LA358_RS00050 (LA358_00050)	8352..8687	+	336	WP_042070764.1	hypothetical protein	-
LA358_RS00055 (LA358_00055)	8731..9129	+	399	WP_224210504.1	hypothetical protein	-
LA358_RS00060 (LA358_00060)	9152..9711	+	560	Protein_11	GNAT family N-acetyltransferase	-
LA358_RS00065 (LA358_00065)	9831..10817	-	987	WP_224210505.1	HipA domain-containing protein	Toxin
LA358_RS00070 (LA358_00070)	10868..11182	-	315	WP_124243609.1	HipA N-terminal domain-containing protein	Antitoxin
LA358_RS00075 (LA358_00075)	11167..11493	-	327	WP_224210506.1	helix-turn-helix transcriptional regulator	-
LA358_RS00080 (LA358_00080)	12299..13435	-	1137	WP_224210507.1	2-iminoacetate synthase ThiH	-
LA358_RS00085 (LA358_00085)	13546..14313	-	768	WP_224210508.1	thiazole synthase	-
LA358_RS00090 (LA358_00090)	14315..14521	-	207	WP_224210509.1	sulfur carrier protein ThiS	-
LA358_RS00095 (LA358_00095)	14518..15306	-	789	WP_224210510.1	HesA/MoeB/ThiF family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (39-287)

Antitoxin

• HipA, N-terminal subdomain 1 (6-98)

Sequences

Toxin

Protein

• Length: 329 a.a.
• Molecular weight: 37359.70 Da
• Isoelectric Point: 6.0542

>T216855 WP_224210505.1 NZ_CP083612:c10817-9831 [Aeromonas enteropelogenes]
MEYSSAGLKKLTGTSRANPSLGFTREQFVHELPLAQKGMSISGYQPKLQMVLKDNEFSVVDHQGDYILKPSPSEFPYLAE
NEHATMTVMERLKFDVPPHGLLSFKKENPDDELEFAFIIKRFDRCENTGNPIHQEQLDGAMGISDKFGKTKSDGKQYVSY
EQVIKFLINSIDDNVKFKIELFRRIAYAYLLGNNDMHLRNFGIIHPSTGKPHLSPVYDFVSVAPYNAYFSSCYLALPLLV
KEEGDKELAFGFKTEYGEYIGVDFLELGQNMGLSPKLTEKLITDLKNESHIVESTYKDSFMPAQAIEDTLRCYRSRLERI
LVFKAALI

Nucleotide

• Length: 987 bp

>T216855 NZ_CP113493:4631992-4632366 [Escherichia coli]
ATGAAAACATTATCCGACACACATGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTACGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCCCTCAGTTAATCAACAGCATTGATATTCTCCGGGCACGCCGGGCAACAGGTCTGATGACCCGCAG
CAACTACAGAACGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12041.64 Da
• Isoelectric Point: 5.6490

>AT216855 WP_124243609.1 NZ_CP083612:c11182-10868 [Aeromonas enteropelogenes]
MRRQVEVYLYGKHIGLLRQNDDGYLFEYNNGYMGPPLSLSFPVNQGRFYSDSLHPYFASLAPEGWLRKRYSQLQQLDEND
LFGILIQNGKNLIGAVQLIVENEV

Nucleotide

• Length: 315 bp

>AT216855 NZ_CP113493:4631571-4631945 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGCACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGGGCACGTCTGGTACAGGAAGGCAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCGGT
TCAGCGACGCAGATGCATACCATCCGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGTTACGTTTATCTGGCGGTTTATCCGACACCGGCACCCGCAACCACCTCATAA