Detailed information of TA system TA216852

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 65679..66406
• Replicon: plasmid pVir-fusion
• Accession: NZ_CP083611
• Organism: Escherichia coli strain EC2648-Vir1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: LAJ48_RS22645
• Protein ID: WP_011251285.1
• Coordinates: 66095..66406 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LAJ48_RS22640
• Protein ID: WP_011251286.1
• Coordinates: 65679..66098 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LAJ48_RS22610 (LAJ48_22620)	60847..61317	-	471	WP_048333570.1	hypothetical protein	-
LAJ48_RS22615 (LAJ48_22625)	61630..62265	-	636	WP_223171879.1	hypothetical protein	-
LAJ48_RS22620 (LAJ48_22630)	62684..63304	+	621	WP_223175004.1	conjugative transfer protein MobI(A/C)	-
LAJ48_RS22625 (LAJ48_22635)	63325..64113	+	789	WP_040217257.1	hypothetical protein	-
LAJ48_RS22630 (LAJ48_22640)	64127..64492	+	366	WP_048333448.1	hypothetical protein	-
LAJ48_RS22635 (LAJ48_22645)	64564..65532	-	969	WP_074428168.1	IS5 family transposase	-
LAJ48_RS22640 (LAJ48_22650)	65679..66098	-	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
LAJ48_RS22645 (LAJ48_22655)	66095..66406	-	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
LAJ48_RS22650 (LAJ48_22660)	66611..67048	-	438	Protein_64	DDE-type integrase/transposase/recombinase	-
LAJ48_RS22655 (LAJ48_22665)	67183..67880	+	698	WP_223175001.1	IS1-like element IS1A family transposase	-
LAJ48_RS22660 (LAJ48_22670)	67882..68331	+	450	WP_011251283.1	thioredoxin fold domain-containing protein	-
LAJ48_RS22665 (LAJ48_22675)	68348..68659	+	312	WP_011251282.1	hypothetical protein	-
LAJ48_RS22670 (LAJ48_22680)	68673..69014	+	342	WP_011251281.1	hypothetical protein	-
LAJ48_RS22675 (LAJ48_22685)	69074..70030	+	957	WP_011251280.1	DsbA family protein	-
LAJ48_RS22680 (LAJ48_22690)	70455..70895	-	441	WP_011251275.1	hypothetical protein	-
LAJ48_RS22685 (LAJ48_22695)	70901..71395	-	495	WP_011251274.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..255611	255611
	-	inside	IScluster/Tn	-	-	49008..73315	24307

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T216852 WP_011251285.1 NZ_CP083611:c66406-66095 [Escherichia coli]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T216852 NZ_CP113493:c4285861-4285550 [Escherichia coli]
ATGCACATCATTTCGAAAGCGCCATTTGAAGAGTGTGCGCGCAAATATCCTAACGACGCTCTGGCTCTACACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCTAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT216852 WP_011251286.1 NZ_CP083611:c66098-65679 [Escherichia coli]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT216852 NZ_CP113493:c4285553-4285134 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCAGCAATGCCGGTT
GGCGTGGCCTTACTTCGTACGCTGATTGATCAACATAATTTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
TCTGGTCAGTCAGATTTTGTCTGGGCAACGATCCCTGACGATATCCCATATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1