Detailed information of TA system TA216825

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symER/SymE(toxin)
• Location: 783282..783694
• Replicon: chromosome
• Accession: NZ_CP083610
• Organism: Escherichia coli strain EC2648-Vir1

Toxin (Protein)

• Gene name: symE
• Uniprot ID: U9YSY7
• Locus tag: LAJ48_RS03845
• Protein ID: WP_000132601.1
• Coordinates: 783353..783694 (+)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	symR
Locus tag	-
Coordinates	783282..783358 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LAJ48_RS03835 (780145)	780145..781734	+	1590	WP_001063204.1	type I restriction-modification system methyltransferase	-
LAJ48_RS03840 (781731)	781731..783125	+	1395	WP_072647344.1	type I restriction-modification system specificity subunit	-
- (783282)	783282..783358	-	77	NuclAT_14	-	Antitoxin
- (783282)	783282..783358	-	77	NuclAT_14	-	Antitoxin
- (783282)	783282..783358	-	77	NuclAT_14	-	Antitoxin
- (783282)	783282..783358	-	77	NuclAT_14	-	Antitoxin
- (783282)	783282..783358	-	77	NuclAT_15	-	Antitoxin
- (783282)	783282..783358	-	77	NuclAT_15	-	Antitoxin
- (783282)	783282..783358	-	77	NuclAT_15	-	Antitoxin
- (783282)	783282..783358	-	77	NuclAT_15	-	Antitoxin
LAJ48_RS03845 (783353)	783353..783694	+	342	WP_000132601.1	endoribonuclease SymE	Toxin
LAJ48_RS03850 (783856)	783856..785235	+	1380	WP_072647343.1	5-methylcytosine-specific restriction endonuclease subunit McrB	-
LAJ48_RS03855 (785235)	785235..786281	+	1047	WP_000437621.1	5-methylcytosine-specific restriction endonuclease system specificity protein McrC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12203.02 Da
• Isoelectric Point: 8.5012

>T216825 WP_000132601.1 NZ_CP083610:783353-783694 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRHVTVGYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAEESE
LMQSLRQVCKLSARKQKQVQAFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T216825 NZ_CP113486:c4847117-4846623 [Escherichia coli]
ATGATTCAGGCCCCCGAGCCACTCATGGCAAGGCATCAATTCACCTCTTTTTGCTCGGGAGTAGAAACGATGGATAACTG
GCTAAAGCAGAGAGCCCTAAAAAATCAGCTTGCCGGCGCATCACGTACATTTGTTAGCTGTGATACGTATAGCAACGTTT
TGGCATATTATTCGCTTGCTTCCAGCGCCGTTGAAACGTATGTCGCGACAGGACGATTTCGCCGAAATATGCCAGAACCG
ATCCCTGTTGTAGTACTTGGTCGTCTGGCAATAGATAAATCATTACAGGGTCAGGGCATCGGTCGTGCGATGGTCAGAGA
TGCCGGTCTACGAGTACTACAGGCGGCTGAAGTCATTGGTATTCGCGGAATGTTGGTCCATGCGTTATCAGACCAGGCCC
GTGAGTTTTATCTGCGAGTGGGGTTTGAACCATCGCCTGTCGATTCTATGATACTGATGGCAACATTGGCTGATTTGCAG
GAATGTCTTAAATAG

Antitoxin

Nucleotide

• Length: 77 bp

>AT216825 NZ_CP083610:c783358-783282 [Escherichia coli]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: U9YSY7

Antitoxin