Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 468842..469536 Replicon chromosome
Accession NZ_CP083610
Organism Escherichia coli strain EC2648-Vir1

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag LAJ48_RS02365 Protein ID WP_001263489.1
Coordinates 468842..469240 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag LAJ48_RS02370 Protein ID WP_000554758.1
Coordinates 469243..469536 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (464430) 464430..464510 - 81 NuclAT_12 - -
- (464430) 464430..464510 - 81 NuclAT_12 - -
- (464430) 464430..464510 - 81 NuclAT_12 - -
- (464430) 464430..464510 - 81 NuclAT_12 - -
LAJ48_RS02340 (465106) 465106..465564 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
LAJ48_RS02345 (465825) 465825..467282 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
LAJ48_RS02350 (467339) 467339..467860 - 522 Protein_464 peptide chain release factor H -
LAJ48_RS02355 (467856) 467856..468062 - 207 Protein_465 RtcB family protein -
LAJ48_RS02360 (468380) 468380..468832 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
LAJ48_RS02365 (468842) 468842..469240 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
LAJ48_RS02370 (469243) 469243..469536 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
LAJ48_RS02375 (469588) 469588..470643 - 1056 WP_001226164.1 DNA polymerase IV -
LAJ48_RS02380 (470714) 470714..471499 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
LAJ48_RS02385 (471471) 471471..473183 + 1713 Protein_471 flagellar biosynthesis protein FlhA -
LAJ48_RS02390 (473407) 473407..473904 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T216823 WP_001263489.1 NZ_CP083610:c469240-468842 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T216823 NZ_CP113486:c4137941-4137723 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT216823 WP_000554758.1 NZ_CP083610:c469536-469243 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT216823 NZ_CP113486:c4138341-4137967 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References