Detailed information of TA system TA216819

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 1079147..1079669
• Replicon: chromosome
• Accession: NZ_CP083608
• Organism: Macrococcus armenti strain JEK37

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: LAU42_RS05555
• Protein ID: WP_224184682.1
• Coordinates: 1079147..1079419 (-)
• Length: 91 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: LAU42_RS05560
• Protein ID: WP_224184683.1
• Coordinates: 1079412..1079669 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LAU42_RS05515 (LAU42_05515)	1074514..1074972	+	459	WP_224184676.1	NUDIX domain-containing protein	-
LAU42_RS05520 (LAU42_05520)	1075064..1075315	+	252	WP_086042489.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
LAU42_RS05525 (LAU42_05525)	1075315..1075575	+	261	WP_133418826.1	Txe/YoeB family addiction module toxin	-
LAU42_RS05530 (LAU42_05530)	1075576..1076064	-	489	WP_224184677.1	GNAT family N-acetyltransferase	-
LAU42_RS05535 (LAU42_05535)	1076108..1076848	+	741	WP_224184678.1	hypothetical protein	-
LAU42_RS05540 (LAU42_05540)	1076858..1077748	+	891	WP_224184679.1	phosphotransferase	-
LAU42_RS05545 (LAU42_05545)	1077872..1078357	+	486	WP_224184680.1	NUDIX domain-containing protein	-
LAU42_RS05550 (LAU42_05550)	1078363..1079124	+	762	WP_224184681.1	hypothetical protein	-
LAU42_RS05555 (LAU42_05555)	1079147..1079419	-	273	WP_224184682.1	Txe/YoeB family addiction module toxin	Toxin
LAU42_RS05560 (LAU42_05560)	1079412..1079669	-	258	WP_224184683.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LAU42_RS05565 (LAU42_05565)	1079941..1080471	+	531	WP_224184684.1	GrpB family protein	-
LAU42_RS05570 (LAU42_05570)	1080968..1082230	+	1263	WP_224184685.1	Y-family DNA polymerase	-
LAU42_RS05575 (LAU42_05575)	1082264..1082368	+	105	WP_224184686.1	DUF4298 domain-containing protein	-
LAU42_RS05580 (LAU42_05580)	1082575..1084095	+	1521	WP_224184687.1	ABC-F family ATP-binding cassette domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (8-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-69)

Sequences

Toxin

Protein

• Length: 91 a.a.
• Molecular weight: 10720.54 Da
• Isoelectric Point: 9.7524

>T216819 WP_224184682.1 NZ_CP083608:c1079419-1079147 [Macrococcus armenti]
MPKKKILFYSSGFEDYTYWQTNDKTVLKKINKLIKSIMIDGANEGIGKPEKLLGNYTGCYSRRITHEHRLVYKIEDDLIC
ILQCRFHYKR

Nucleotide

• Length: 273 bp

>T216819 NZ_CP113486:3507978-3508355 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCAGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGGCCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGTACCGACCTGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGATCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9708.82 Da
• Isoelectric Point: 4.2404

>AT216819 WP_224184683.1 NZ_CP083608:c1079669-1079412 [Macrococcus armenti]
MKVTTYSNARSNFRKIIDIVNEDCEPYTITTNTHNAVILSEQDYNSMIETLYLQSTPANAERLQKAIDESINKNNLLQVE
IDLDA

Nucleotide

• Length: 258 bp

>AT216819 NZ_CP113486:3507520-3507888 [Escherichia coli]
GTGTCAGACACACTCTCCGGGACAACGCATCCCGATGACAATCACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA