Detailed information of TA system TA216810

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 1117478..1118000
• Replicon: chromosome
• Accession: NZ_CP083598
• Organism: Macrococcus armenti strain JEK12

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: LAU44_RS05850
• Protein ID: WP_224186318.1
• Coordinates: 1117478..1117750 (-)
• Length: 91 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: LAU44_RS05855
• Protein ID: WP_224186319.1
• Coordinates: 1117743..1118000 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LAU44_RS05815 (LAU44_05815)	1113395..1113646	+	252	WP_086042489.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
LAU44_RS05820 (LAU44_05820)	1113646..1113906	+	261	WP_133418826.1	Txe/YoeB family addiction module toxin	-
LAU44_RS05825 (LAU44_05825)	1113907..1114356	-	450	WP_224202679.1	GNAT family N-acetyltransferase	-
LAU44_RS05830 (LAU44_05830)	1114439..1115179	+	741	WP_224186314.1	hypothetical protein	-
LAU44_RS05835 (LAU44_05835)	1115189..1116079	+	891	WP_224186315.1	phosphotransferase	-
LAU44_RS05840 (LAU44_05840)	1116203..1116688	+	486	WP_224186316.1	NUDIX domain-containing protein	-
LAU44_RS05845 (LAU44_05845)	1116694..1117455	+	762	WP_224186317.1	hypothetical protein	-
LAU44_RS05850 (LAU44_05850)	1117478..1117750	-	273	WP_224186318.1	Txe/YoeB family addiction module toxin	Toxin
LAU44_RS05855 (LAU44_05855)	1117743..1118000	-	258	WP_224186319.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LAU44_RS05860 (LAU44_05860)	1118266..1118796	+	531	WP_224186320.1	GrpB family protein	-
LAU44_RS12425	1118923..1119048	+	126	WP_277602267.1	hypothetical protein	-
LAU44_RS05865 (LAU44_05865)	1119084..1120697	+	1614	WP_224186321.1	ABC-F type ribosomal protection protein	-
LAU44_RS05870 (LAU44_05870)	1121037..1122299	+	1263	WP_224186322.1	Y-family DNA polymerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (6-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-69)

Sequences

Toxin

Protein

• Length: 91 a.a.
• Molecular weight: 10678.50 Da
• Isoelectric Point: 9.8923

>T216810 WP_224186318.1 NZ_CP083598:c1117750-1117478 [Macrococcus armenti]
MPKKKILFYSGGFEDYTYWQTNDKTVLKKIKKLIKSIMIDGANEGIGKPEKLLGNYTGCYSRRITHEHRLVYKIEDDSIC
ILQCRFHYKR

Nucleotide

• Length: 273 bp

>T216810 NZ_CP113486:2153639-2154103 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTACAGCGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAGCCCATTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9739.83 Da
• Isoelectric Point: 4.1540

>AT216810 WP_224186319.1 NZ_CP083598:c1118000-1117743 [Macrococcus armenti]
MKVTTYSNARSNFRKIIDIVNEDCEPYTITTNTHNAVILSEQDYNSMIETLYLQSTPTNAERLQKAIDESIDKNNLLQVE
IDLDA

Nucleotide

• Length: 258 bp

>AT216810 NZ_CP113486:2153304-2153639 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCGACACTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA