Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 635374..636101 Replicon chromosome
Accession NZ_CP083585
Organism Escherichia coli strain ECNB21-M121

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag LA372_RS03190 Protein ID WP_000550189.1
Coordinates 635374..635688 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag LA372_RS03195 Protein ID WP_001514564.1
Coordinates 635685..636101 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LA372_RS03170 (631532) 631532..632518 - 987 WP_001718894.1 Gfo/Idh/MocA family oxidoreductase -
LA372_RS03175 (632597) 632597..633289 - 693 WP_047623784.1 vancomycin high temperature exclusion protein -
LA372_RS03180 (633378) 633378..633869 - 492 WP_224204982.1 M48 family metallopeptidase -
LA372_RS03185 (633954) 633954..635090 + 1137 WP_024250066.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
LA372_RS03190 (635374) 635374..635688 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
LA372_RS03195 (635685) 635685..636101 + 417 WP_001514564.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
LA372_RS03200 (636146) 636146..638164 - 2019 WP_047623783.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
LA372_RS03205 (638490) 638490..640841 - 2352 WP_224204983.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-136)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T216772 WP_000550189.1 NZ_CP083585:635374-635688 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T216772 NZ_CP113432:c66516-66235 [Pseudomonas sp. ZM23]
ATGAGCCTGCAGTGGACGCACAAGGCGGCTGCCGACCTGGACGCCATCTACGACCACTACGTAGTGCTTATCGGCCCGGA
GAAAGCGCTGCGAGCCATCCAGGACATCGTCGAGCAGGTGCGCCCGCTGATGAACCTGGAGCAAAGCAGCAGCGGCGCGC
CCAGCGAGGTGCCAGGTGTGCGCGAACTATCTATCGAGCGCTGGCCGTACCAGGCCGCATTCCGGGTCAAGGGGCGTTCT
GTACAGATTTTGCGCATCGACCGTGTGGATAACTCGGGGTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15025.44 Da        Isoelectric Point: 4.4547

>AT216772 WP_001514564.1 NZ_CP083585:635685-636101 [Escherichia coli]
MITIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT216772 NZ_CP113432:c66740-66513 [Pseudomonas sp. ZM23]
TTGACCACCGTTGTTTCCTTCCGCGCGGATGACGCCCTTGTCGAAGCGCTGGACCAGTTGGCCCGTGCCACCCATCGCGA
CCGCCCGTATCACCTGCGCCAGGCCCTGGCGCAGTACCTGGAGCGGCAGAGCTGGCACGTGGCGACGATCGATGAAGGCG
TGGCGGATGCCGATGCCGGCCACCTGCTGGAGCACACCGTCATCGAAGCGAAATGGGGGCTGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References