Detailed information of TA system TA216771

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 592258..593057
• Replicon: chromosome
• Accession: NZ_CP083585
• Organism: Escherichia coli strain ECNB21-M121

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: -
• Locus tag: LA372_RS02965
• Protein ID: WP_047623792.1
• Coordinates: 592258..592722 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: LA372_RS02970
• Protein ID: WP_001307405.1
• Coordinates: 592722..593057 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LA372_RS02935 (587259)	587259..587693	-	435	WP_000948824.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-
LA372_RS02940 (587711)	587711..588589	-	879	WP_001295548.1	PTS N-acetylgalactosamine transporter subunit IID	-
LA372_RS02945 (588579)	588579..589358	-	780	WP_047623793.1	PTS N-acetylgalactosamine transporter subunit IIC	-
LA372_RS02950 (589369)	589369..589842	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
LA372_RS02955 (589865)	589865..591145	-	1281	WP_000681939.1	tagatose-bisphosphate aldolase subunit KbaZ	-
LA372_RS02960 (591394)	591394..592203	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
LA372_RS02965 (592258)	592258..592722	-	465	WP_047623792.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
LA372_RS02970 (592722)	592722..593057	-	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
LA372_RS02975 (593207)	593207..594778	-	1572	WP_001273763.1	galactarate dehydratase	-
LA372_RS02980 (595153)	595153..596487	+	1335	WP_000599633.1	galactarate/glucarate/glycerate transporter GarP	-
LA372_RS02985 (596503)	596503..597273	+	771	WP_024250085.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17811.16 Da
• Isoelectric Point: 9.4947

>T216771 WP_047623792.1 NZ_CP083585:c592722-592258 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEAFKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Nucleotide

• Length: 465 bp

>T216771 NZ_CP113428:2341674-2342411 [Bacillus cereus]
GTGATCTCTAACATTCGAATCGGCTTATTTATTTTAGCAATCGTTTTTCTAATACTTGTTTTCTTTTATTGGAAAAACGA
AGAGTTGTACGAGGAGAAGAAGCAACGTATTAGAAAGACTTGGTATGGGTTGTTTATAATATCAGTCACTGTGTATTTCA
TGATAAAAGGAATTGATTTAACCCTCTGGAAAAATCTTTTAATGTTTACTGCAATGGTTATTTTCGTTGATATTGCATTT
ATTTTAACACCTAATATTTCAGAAATATGGGGAGCGAAATTTAGCGATATCGGCAAGACGGTTCAATCGATAAAACGGTC
ATTAATTGCTTCTAAAGCAAGAGGAGAAATATACACAACAATCATTCAAAATGTCAATCCAGCAGTGTTCGGTACGATGG
AATGGCATACGGAGGAAGAATATACAAAAAGCTTAAACGCATTTTTAGATTCGTATGGGGAAAAGATTGGAGCAAAAATT
GTTGTATTTGAAGCGGCAAAGGAATTAAATACAAACTTTCGTGGTATCCGTTCTCAATTTAGTACGATTATTCCGTTAGA
GTACATTGAGCAATTGAATGAGCAGAGAGCAGTACAAGTAGAAAATGTCGGAATTATACCAGCAAAAATAGTGAGTGATG
TTTTCATTGTTATTGATGGGAAGAAAAATAACCTGCAAGATCGAGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT216771 WP_001307405.1 NZ_CP083585:c593057-592722 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT216771 NZ_CP113428:2342524-2342649 [Bacillus cereus]
GTGATAAAAGATTATGACAATGATTTTTTTAAAGAAGATAATGAAGATACAACAGATTTCTCGTTAATAAAAGGAGATAC
ATTACAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0XVC7