Detailed information of TA system TA021677

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1775885..1776510
• Replicon: chromosome
• Accession: NC_008314
• Organism: Ralstonia eutropha H16

Toxin (Protein)

• Gene name: graT
• Uniprot ID: R7XAD8
• Locus tag: H16_RS26575
• Protein ID: WP_010814558.1
• Coordinates: 1775885..1776163 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H16_RS26580
• Protein ID: WP_010814559.1
• Coordinates: 1776175..1776510 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H16_RS26575	1775885..1776163	+	279	WP_010814558.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H16_RS26580	1776175..1776510	+	336	WP_010814559.1	HigA family addiction module antidote protein	Antitoxin
H16_RS26585	1776562..1777932	-	1371	WP_011617316.1	serine hydrolase	-
H16_RS26590	1777979..1778458	-	480	WP_010814561.1	MarR family transcriptional regulator	-
H16_RS26595	1778637..1779233	-	597	WP_010814562.1	GTP cyclohydrolase II	-
H16_RS26600	1779502..1780032	+	531	WP_010814563.1	hypothetical protein	-
H16_RS26605	1780041..1780511	-	471	WP_010814564.1	Lrp/AsnC family transcriptional regulator	-
H16_RS26610	1780663..1781004	+	342	WP_010814565.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (24-68)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10732.42 Da
• Isoelectric Point: 10.4151

>T21677 WP_010814558.1 NC_008314:1775885-1776163 [Cupriavidus necator H16]
MIQSFRCSDTAELFAGRCVPRFGNIRAVAERKLRLLQRASTLEFLRSPPGNRLQLLTGDRKGRYSIRINEQWRICFIWTH
DGPAVVEIIDYH

Nucleotide

• Length: 279 bp

>T21677 NC_008314:1775885-1776163 [Cupriavidus necator H16]
ATGATTCAGTCATTCAGATGTTCCGACACCGCTGAACTGTTCGCGGGCCGCTGTGTACCGCGCTTCGGCAATATCCGCGC
CGTGGCCGAACGCAAGCTGCGGCTGCTGCAGAGAGCGTCGACGCTGGAGTTCCTGCGCTCCCCGCCGGGCAATCGCCTGC
AGTTGCTGACCGGCGACCGCAAAGGCCGCTACAGCATCCGCATCAACGAGCAATGGCGAATCTGCTTCATCTGGACCCAC
GACGGCCCCGCGGTCGTTGAGATCATCGACTACCACTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12308.11 Da
• Isoelectric Point: 7.2042

>AT21677 WP_010814559.1 NC_008314:1776175-1776510 [Cupriavidus necator H16]
MVRFKDGMRPIHPGEILREDFLVPLNMSANALALALRVTAARINDIVREQRGITPDTALRLARYFGGDAQSWLNLQLDYD
LKVAQRDHGERIMAEVAPRDAAAAGQPAAEK

Nucleotide

• Length: 336 bp

>AT21677 NC_008314:1776175-1776510 [Cupriavidus necator H16]
ATGGTTCGCTTCAAGGACGGCATGCGTCCCATCCACCCGGGCGAAATCCTGCGCGAGGATTTCCTGGTACCGCTCAACAT
GAGCGCCAACGCCCTGGCCCTCGCGCTGCGCGTGACCGCGGCGCGCATCAACGACATCGTCCGCGAGCAACGTGGCATCA
CGCCGGACACCGCCCTGCGCCTGGCGCGCTATTTTGGCGGCGATGCGCAGTCGTGGCTGAACCTGCAGCTGGACTATGAC
CTGAAGGTCGCGCAGCGCGACCACGGCGAGCGCATCATGGCGGAAGTGGCGCCGCGCGATGCCGCGGCGGCCGGTCAACC
GGCCGCTGAAAAGTAG

Structures

Toxin

• AlphaFold DB: A0A7T4G2P6