Detailed information of TA system TA216751

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 129217..129638
• Replicon: plasmid unnamed1
• Accession: NZ_CP083531
• Organism: Escherichia coli strain elppa2

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: K9U05_RS24130
• Protein ID: WP_096937776.1
• Coordinates: 129217..129342 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	129440..129638 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9U05_RS24090 (124280)	124280..124495	-	216	WP_001352842.1	conjugal transfer relaxosome protein TraY	-
K9U05_RS24095 (124631)	124631..125278	-	648	WP_000332520.1	conjugal transfer transcriptional regulator TraJ	-
K9U05_RS24100 (125470)	125470..125853	-	384	WP_001063020.1	conjugal transfer relaxosome DNA-binding protein TraM	-
K9U05_RS24105 (126205)	126205..126795	+	591	WP_166494936.1	transglycosylase SLT domain-containing protein	-
K9U05_RS24110 (127092)	127092..127913	-	822	WP_001234475.1	DUF932 domain-containing protein	-
K9U05_RS24115 (128032)	128032..128319	-	288	WP_000107546.1	hypothetical protein	-
K9U05_RS24120 (128344)	128344..128550	-	207	WP_228613817.1	single-stranded DNA-binding protein	-
K9U05_RS24125 (128620)	128620..128916	+	297	Protein_128	hypothetical protein	-
K9U05_RS24130 (129217)	129217..129342	-	126	WP_096937776.1	type I toxin-antitoxin system Hok family toxin	Toxin
K9U05_RS24135 (129284)	129284..129433	-	150	Protein_130	DUF5431 family protein	-
- (129440)	129440..129638	-	199	NuclAT_0	-	Antitoxin
- (129440)	129440..129638	-	199	NuclAT_0	-	Antitoxin
- (129440)	129440..129638	-	199	NuclAT_0	-	Antitoxin
- (129440)	129440..129638	-	199	NuclAT_0	-	Antitoxin
K9U05_RS24140 (129607)	129607..130369	-	763	Protein_131	plasmid SOS inhibition protein A	-
K9U05_RS24145 (130366)	130366..130800	-	435	WP_000845928.1	conjugation system SOS inhibitor PsiB	-
K9U05_RS24150 (130855)	130855..132813	-	1959	WP_029487628.1	ParB/RepB/Spo0J family partition protein	-
K9U05_RS24155 (132879)	132879..133112	-	234	WP_000005990.1	DUF905 family protein	-
K9U05_RS24160 (133169)	133169..133708	-	540	WP_000290801.1	single-stranded DNA-binding protein	-
K9U05_RS24165 (134026)	134026..134535	+	510	WP_071600123.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD / dfrA14	iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroE / iroN / vat	1..155994	155994

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4806.72 Da
• Isoelectric Point: 8.4890

>T216751 WP_096937776.1 NZ_CP083531:c129342-129217 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T216751 NZ_CP113364:286483-287001 [Salmonella enterica subsp. enterica]
GTGGACAACCTGACAATAGAGATTCTGGCTGATAACGCAGAATACAACTGGCGGCAGTTCGACTGCGGTGAAGCATCATT
AAACCTTTTTTTAACGCAGCATCTTCAGCGCCAGCATAACAACAAAATCTTGCGCGGTTATGTACTCAGAACCACTACGC
CAGAACGGCGAGTGTTGGGGTATTACACCCTGTCAGGAAGCTGCTTTGAACGCGCGTCGTTGCCTTCCAGAACCCAGCAA
AAGCGCATCCCTTACCAGAATATCCCCAGCGTTACGCTGGGCCGTTTAGCGGTCGATCTCTCATTACAGGGAAAAGGGTG
GGGAGCCATATTGGTCACGCATGCGATGAAAGTGGTGTGGTCGGCATCACTGGCCGTTGGGATTCATGGCATCTTTGTGG
AAGCGTTAAACGAAAAAGCCCAGGCCTTTTATCAGCGGCTGGGCTTTATTTCTTTATCCGGAGAAAACGAGCACGCGCTG
TTTTACCCGACAAAGTCGATTGAGCAACTTTTTGGCTAG

Antitoxin

Nucleotide

• Length: 199 bp

>AT216751 NZ_CP083531:c129638-129440 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin