Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 2973370..2974064 Replicon chromosome
Accession NZ_CP083530
Organism Escherichia coli strain elppa2

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag K9U05_RS13880 Protein ID WP_001263491.1
Coordinates 2973666..2974064 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag K9U05_RS13875 Protein ID WP_000554755.1
Coordinates 2973370..2973663 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9U05_RS13850 (2968406) 2968406..2968666 - 261 WP_000729708.1 type II toxin-antitoxin system antitoxin DinJ -
K9U05_RS13855 (2968852) 2968852..2969625 + 774 WP_022645219.1 C40 family peptidase -
K9U05_RS13860 (2969723) 2969723..2971435 - 1713 Protein_2692 flagellar biosynthesis protein FlhA -
K9U05_RS13865 (2971407) 2971407..2972192 + 786 WP_000207551.1 putative lateral flagellar export/assembly protein LafU -
K9U05_RS13870 (2972263) 2972263..2973318 + 1056 Protein_2694 DNA polymerase IV -
K9U05_RS13875 (2973370) 2973370..2973663 + 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
K9U05_RS13880 (2973666) 2973666..2974064 + 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
K9U05_RS13885 (2974074) 2974074..2974358 + 285 Protein_2697 GNAT family N-acetyltransferase -
K9U05_RS13895 (2975127) 2975127..2975303 + 177 Protein_2699 GNAT family N-acetyltransferase -
K9U05_RS13900 (2975494) 2975494..2976633 + 1140 WP_022645224.1 RNA ligase RtcB family protein -
K9U05_RS13905 (2976630) 2976630..2977244 + 615 WP_022645225.1 peptide chain release factor H -
K9U05_RS13910 (2977306) 2977306..2977815 + 510 Protein_2702 metal-dependent hydrolase -
K9U05_RS13915 (2977832) 2977832..2978113 - 282 WP_022645226.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2974374..2974751 377


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T216727 WP_001263491.1 NZ_CP083530:2973666-2974064 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T216727 NZ_CP113242:c248346-248080 [Vibrio sp. gvc]
ATGATTAAATTTGAATTTGATGAAAACAAAAGCAGAAGTAATCTCGAAAAACATGGTATCGATTTTCATACCGCTCAAGG
ATTGTGGAATGATTCAGATCTTATCGAGATCCCTGCTAATACGAGTGATGAACCAAGATATTTGGTTGTCGGCTTACTAA
ACGGTAAGCATTGGTCAGGTGTCATTACCTACCGAGGCATGAATATCAGGCTCATCTCAGTTCGGCGTTCACGGAAAGCG
GAGATAGACCTTTATGAAAGCGCATGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT216727 WP_000554755.1 NZ_CP083530:2973370-2973663 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT216727 NZ_CP113242:c248093-247848 [Vibrio sp. gvc]
ATGAAAGCGCATGAATTTGATGCTAAGTTTGAAAGTGACGACGATGACATCTTAATGGATTTGGATTTGTCGCAAGTAAA
AAGGCCAATGCATAAGCAAAAACGAGTCAATGTCGATTTTCCAGCGTGGATGCTTGAGTCTTTAGACCGAGAAGCCAATC
GTGTAGGTGTAACACGCCAATCAATCATTAAAATTTGGCTAGCAGAAAGGCTAGAAAGTGTTTCTCATCATGCAAGTGTG
AGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References