Detailed information of TA system TA216716

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 1202555..1203354
• Replicon: chromosome
• Accession: NZ_CP083530
• Organism: Escherichia coli strain elppa2

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: K9U05_RS05570
• Protein ID: WP_000347273.1
• Coordinates: 1202890..1203354 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: D6JF08
• Locus tag: K9U05_RS05565
• Protein ID: WP_001308975.1
• Coordinates: 1202555..1202890 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9U05_RS05550 (1198340)	1198340..1199110	-	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-
K9U05_RS05555 (1199126)	1199126..1200460	-	1335	WP_000599651.1	galactarate/glucarate/glycerate transporter GarP	-
K9U05_RS05560 (1200835)	1200835..1202406	+	1572	WP_077516436.1	galactarate dehydratase	-
K9U05_RS05565 (1202555)	1202555..1202890	+	336	WP_001308975.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
K9U05_RS05570 (1202890)	1202890..1203354	+	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
K9U05_RS05575 (1203409)	1203409..1204218	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
K9U05_RS05580 (1204467)	1204467..1205747	+	1281	Protein_1090	tagatose-bisphosphate aldolase subunit KbaZ	-
K9U05_RS05585 (1205770)	1205770..1206243	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
K9U05_RS05590 (1206254)	1206254..1207033	+	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
K9U05_RS05595 (1207023)	1207023..1207901	+	879	WP_228613213.1	PTS N-acetylgalactosamine transporter subunit IID	-
K9U05_RS05600 (1207919)	1207919..1208353	+	435	WP_000948824.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T216716 WP_000347273.1 NZ_CP083530:1202890-1203354 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T216716 NZ_CP113236:2258825-2259139 [Pasteurella multocida]
ATGCTCGATATAATTGAAACAACAGTATTTAATAAATGGCTTAAAGAGCTAAAGGATTTGTCGGCTAAAGCGGCTATTCT
TGCACGTATTAGTCGCGCTAAAAGTGGTAATTTTGGTGACCATAAATCAGTTGGTGATGGTTTGTATGAAATGCGAATAA
TGAAAGGAGCTGGTTATAGGGTTTATTATGCACAATACAAAGATGTAACCTATTTGCTAATTTGTGGCGGTGATAAATCT
ACTCAGAAAGCAGATATCGCTAAAGCAAAAGCATTATGGGAAGAGATTAAGCAGAAAGAGGAGATAAATGTATGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12343.97 Da
• Isoelectric Point: 4.8616

>AT216716 WP_001308975.1 NZ_CP083530:1202555-1202890 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT216716 NZ_CP113236:2259136-2259426 [Pasteurella multocida]
ATGAGTAAAAAAGTAGAATTAAAACCATTTGATATTGCTGAATACCTAGATAGTGAAGAAATGATTGCGGCATATTTAAG
TGAAATTTTAGAAACAGGTGACACTAATGAATTTATTTCTGCGCTAGGTGATGTCGCAAGAGCGAGAGGAATGACGGAGT
TAGCGGCAAAAACAGGATTAGGAAGAGAAAGTCTCTATAAAACATTATCACACGGAAGTAAGCCGCGTTTTGATACTATA
ATGAAAATTACGCAAGCACTAGGTATTAAACTTGTCCCAACGCATATCTAA

Structures

Toxin

• AlphaFold DB: V0SSH7

Antitoxin

• AlphaFold DB: D6JF08