Detailed information of TA system TA216710

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 75495..75759
• Replicon: plasmid unnamed2
• Accession: NZ_CP083514
• Organism: Escherichia coli strain elppa4

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Y6M3
• Locus tag: K9U07_RS25880
• Protein ID: WP_001331364.1
• Coordinates: 75495..75647 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	75697..75759 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9U07_RS25850 (70772)	70772..72940	+	2169	WP_000698357.1	DotA/TraY family protein	-
K9U07_RS25855 (73014)	73014..73664	+	651	WP_001178506.1	plasmid IncI1-type surface exclusion protein ExcA	-
K9U07_RS25860 (73736)	73736..73945	-	210	WP_000062603.1	HEAT repeat domain-containing protein	-
K9U07_RS25865 (74337)	74337..74513	+	177	WP_001054897.1	hypothetical protein	-
K9U07_RS25870 (74578)	74578..74810	-	233	Protein_89	hypothetical protein	-
K9U07_RS25875 (75172)	75172..75423	+	252	WP_001291964.1	hypothetical protein	-
K9U07_RS25880 (75495)	75495..75647	-	153	WP_001331364.1	Hok/Gef family protein	Toxin
- (75697)	75697..75759	+	63	NuclAT_0	-	Antitoxin
- (75697)	75697..75759	+	63	NuclAT_0	-	Antitoxin
- (75697)	75697..75759	+	63	NuclAT_0	-	Antitoxin
- (75697)	75697..75759	+	63	NuclAT_0	-	Antitoxin
K9U07_RS25885 (75939)	75939..77147	+	1209	WP_024171190.1	IncI1-type conjugal transfer protein TrbA	-
K9U07_RS25890 (77166)	77166..78236	+	1071	WP_137653290.1	IncI1-type conjugal transfer protein TrbB	-
K9U07_RS25895 (78229)	78229..80520	+	2292	WP_228587552.1	F-type conjugative transfer protein TrbC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..90907	90907

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5775.13 Da
• Isoelectric Point: 8.7948

>T216710 WP_001331364.1 NZ_CP083514:c75647-75495 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T216710 NZ_CP113231:105678-106040 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAGCAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Nucleotide

• Length: 63 bp

>AT216710 NZ_CP083514:75697-75759 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A5W9M382

Antitoxin