Detailed information of TA system TA021671

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 2661081..2661583
• Replicon: chromosome
• Accession: NC_008278
• Organism: Frankia alni ACN14a

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: Q0RN04
• Locus tag: FRAAL_RS10650
• Protein ID: WP_011603600.1
• Coordinates: 2661081..2661335 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: FRAAL_RS10655
• Protein ID: WP_041939128.1
• Coordinates: 2661332..2661583 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FRAAL_RS10630	2656475..2657203	-	729	WP_041940384.1	GAF domain-containing protein	-
FRAAL_RS10635	2657482..2659455	+	1974	WP_041939126.1	PAS domain S-box protein	-
FRAAL_RS10640	2659608..2660060	-	453	WP_011603598.1	pyridoxamine 5'-phosphate oxidase family protein	-
FRAAL_RS10645	2660266..2661036	+	771	WP_041939127.1	enoyl-CoA hydratase/isomerase family protein	-
FRAAL_RS10650	2661081..2661335	-	255	WP_011603600.1	Txe/YoeB family addiction module toxin	Toxin
FRAAL_RS10655	2661332..2661583	-	252	WP_041939128.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
FRAAL_RS10660	2661744..2664068	+	2325	WP_011603602.1	FdhF/YdeP family oxidoreductase	-
FRAAL_RS10665	2664068..2665000	+	933	WP_041939129.1	formate dehydrogenase accessory sulfurtransferase FdhD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10302.65 Da
• Isoelectric Point: 9.4248

>T21671 WP_011603600.1 NC_008278:c2661335-2661081 [Frankia alni ACN14a]
VKLVWDENAWQDYVWWQTENRKILKRINMLIQDTARNGNDGIGKPEPLKHGFQGYWSRRINDEHRLIYKISDDQIRIAQC
RYHY

Nucleotide

• Length: 255 bp

>T21671 NC_008278:c2661335-2661081 [Frankia alni ACN14a]
GTGAAGCTCGTCTGGGACGAGAACGCGTGGCAGGACTACGTCTGGTGGCAGACGGAGAACCGTAAAATCCTCAAGCGCAT
CAACATGTTGATCCAGGACACAGCCCGGAACGGCAACGACGGCATCGGCAAGCCCGAACCGCTCAAGCATGGCTTCCAGG
GCTACTGGTCTCGGCGCATCAACGATGAACATCGACTGATCTACAAGATCAGTGACGATCAGATTCGCATAGCCCAGTGC
AGGTACCACTACTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9249.38 Da
• Isoelectric Point: 4.6412

>AT21671 WP_041939128.1 NC_008278:c2661583-2661332 [Frankia alni ACN14a]
VKTMSYSESRARYAEVLTAVADDREEIVITRAGHEPVVIVSLDDYQSLKETAYLLRSPENARRLLAAIDRLENGAGTVRD
LAE

Nucleotide

• Length: 252 bp

>AT21671 NC_008278:c2661583-2661332 [Frankia alni ACN14a]
GTGAAGACGATGAGCTACTCGGAGTCACGAGCACGCTATGCCGAGGTCCTCACCGCGGTGGCGGACGACCGGGAGGAGAT
CGTCATCACCCGGGCCGGTCACGAGCCGGTCGTCATCGTCTCTCTGGATGACTACCAGTCCCTCAAGGAGACGGCGTACC
TTCTCCGCAGCCCCGAGAACGCCCGCCGGCTGCTGGCCGCCATCGATCGCCTCGAAAACGGCGCCGGCACCGTACGCGAC
CTCGCCGAGTGA

Structures

Toxin

• AlphaFold DB: Q0RN04