Detailed information of TA system TA216702

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 10122..10543
• Replicon: plasmid unnamed1
• Accession: NZ_CP083513
• Organism: Escherichia coli strain elppa4

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: K9U07_RS24610
• Protein ID: WP_096937776.1
• Coordinates: 10418..10543 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	10122..10320 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9U07_RS24575 (5225)	5225..5734	-	510	WP_071600123.1	hypothetical protein	-
K9U07_RS24580 (6052)	6052..6591	+	540	WP_000290801.1	single-stranded DNA-binding protein	-
K9U07_RS24585 (6648)	6648..6881	+	234	WP_000005990.1	DUF905 family protein	-
K9U07_RS24590 (6947)	6947..8905	+	1959	WP_029487628.1	ParB/RepB/Spo0J family partition protein	-
K9U07_RS24595 (8960)	8960..9394	+	435	WP_000845928.1	conjugation system SOS inhibitor PsiB	-
K9U07_RS24600 (9391)	9391..10153	+	763	Protein_14	plasmid SOS inhibition protein A	-
- (10122)	10122..10320	+	199	NuclAT_0	-	Antitoxin
- (10122)	10122..10320	+	199	NuclAT_0	-	Antitoxin
- (10122)	10122..10320	+	199	NuclAT_0	-	Antitoxin
- (10122)	10122..10320	+	199	NuclAT_0	-	Antitoxin
K9U07_RS24605 (10327)	10327..10476	+	150	Protein_15	DUF5431 family protein	-
K9U07_RS24610 (10418)	10418..10543	+	126	WP_096937776.1	type I toxin-antitoxin system Hok family toxin	Toxin
K9U07_RS24615 (10844)	10844..11141	-	298	Protein_17	hypothetical protein	-
K9U07_RS24620 (11211)	11211..11417	+	207	WP_000547965.1	hypothetical protein	-
K9U07_RS24625 (11442)	11442..11729	+	288	WP_228587539.1	hypothetical protein	-
K9U07_RS24630 (11848)	11848..12669	+	822	WP_001234475.1	DUF932 domain-containing protein	-
K9U07_RS24635 (12966)	12966..13556	-	591	WP_228587542.1	transglycosylase SLT domain-containing protein	-
K9U07_RS24640 (13908)	13908..14291	+	384	WP_072647915.1	conjugal transfer relaxosome DNA-binding protein TraM	-
K9U07_RS24645 (14483)	14483..15130	+	648	WP_000332520.1	conjugal transfer transcriptional regulator TraJ	-
K9U07_RS24650 (15266)	15266..15481	+	216	WP_001352842.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 / dfrA14 / tet(A) / sitABCD	vat / iroN / iroE / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA	1..171115	171115

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4806.72 Da
• Isoelectric Point: 8.4890

>T216702 WP_096937776.1 NZ_CP083513:10418-10543 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T216702 NZ_CP113229:c2704999-2704637 [Streptomyces albidoflavus]
ATGGACGGGGAATGGCAGATCTTCCTGGTTGACGAGGTGCGTGTTTGGCTCGCGTCCCTCGACGGGGCCGCGCACGCACG
AGTGGTACAGGCGCTGGACGTGCTGGCCGAGGAAGGGCCTGCTCTCGGAAGACCGCTTGTGGACACCTTGCGTGGTTCAG
CGGTGGCCAATTTGAAAGAGCTCCGGCCCGGCACGGTCAGAATCCTGTTCGCCTTCGATCCGTGGCGTTCCAGCATCTTG
CTGGTGGCAGGTGACAAGTCCGGGCAGCGGACTGAGTGGTACCAGGAGGCGATCCCCCTGGCCGAGCAGCGCTACGCGCT
TTACTTGAAGGAACGTGAGCGGGAAGAGGGAGGACGACCATGA

Antitoxin

Nucleotide

• Length: 199 bp

>AT216702 NZ_CP083513:10122-10320 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin