Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 4084654..4085348 Replicon chromosome
Accession NZ_CP083512
Organism Escherichia coli strain elppa4

Toxin (Protein)


Gene name yafO Uniprot ID A0A1X0YXS1
Locus tag K9U07_RS19735 Protein ID WP_001263484.1
Coordinates 4084950..4085348 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag K9U07_RS19730 Protein ID WP_000554755.1
Coordinates 4084654..4084947 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9U07_RS19705 (4080275) 4080275..4080631 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
K9U07_RS19710 (4080624) 4080624..4080902 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
K9U07_RS19715 (4081007) 4081007..4082719 - 1713 Protein_3846 flagellar biosynthesis protein FlhA -
K9U07_RS19720 (4082691) 4082691..4083476 + 786 WP_000207554.1 putative lateral flagellar export/assembly protein LafU -
K9U07_RS19725 (4083547) 4083547..4084602 + 1056 WP_001226183.1 DNA polymerase IV -
K9U07_RS19730 (4084654) 4084654..4084947 + 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
K9U07_RS19735 (4084950) 4084950..4085348 + 399 WP_001263484.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
K9U07_RS19740 (4085358) 4085358..4085810 + 453 WP_001059899.1 GNAT family N-acetyltransferase -
K9U07_RS19745 (4086055) 4086055..4086261 + 207 Protein_3852 RtcB family protein -
K9U07_RS19750 (4086257) 4086257..4086609 + 353 Protein_3853 peptide chain release factor H -
K9U07_RS19755 (4086666) 4086666..4088123 - 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
K9U07_RS19760 (4088384) 4088384..4088842 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- (4089438) 4089438..4089518 + 81 NuclAT_8 - -
- (4089438) 4089438..4089518 + 81 NuclAT_8 - -
- (4089438) 4089438..4089518 + 81 NuclAT_8 - -
- (4089438) 4089438..4089518 + 81 NuclAT_8 - -
K9U07_RS19765 (4088934) 4088934..4090178 + 1245 WP_000189541.1 esterase FrsA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15404.81 Da        Isoelectric Point: 7.3840

>T216700 WP_001263484.1 NZ_CP083512:4084950-4085348 [Escherichia coli]
MRVFKTKLICLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T216700 NZ_CP113218:87353-87835 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT216700 WP_000554755.1 NZ_CP083512:4084654-4084947 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT216700 NZ_CP113218:87099-87365 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1X0YXS1

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References