Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 2297676..2298475 Replicon chromosome
Accession NZ_CP083512
Organism Escherichia coli strain elppa4

Toxin (Protein)


Gene name yhaV Uniprot ID A0A7B2BI69
Locus tag K9U07_RS11175 Protein ID WP_000347258.1
Coordinates 2298011..2298475 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID A0A368IY36
Locus tag K9U07_RS11170 Protein ID WP_001604880.1
Coordinates 2297676..2298011 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9U07_RS11155 (2293461) 2293461..2294231 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
K9U07_RS11160 (2294247) 2294247..2295581 - 1335 WP_000599654.1 galactarate/glucarate/glycerate transporter GarP -
K9U07_RS11165 (2295956) 2295956..2297527 + 1572 WP_001273737.1 galactarate dehydratase -
K9U07_RS11170 (2297676) 2297676..2298011 + 336 WP_001604880.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
K9U07_RS11175 (2298011) 2298011..2298475 + 465 WP_000347258.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
K9U07_RS11180 (2298530) 2298530..2299339 - 810 WP_000072171.1 aga operon transcriptional regulator AgaR -
K9U07_RS11185 (2299588) 2299588..2300868 + 1281 WP_000681943.1 tagatose-bisphosphate aldolase subunit KbaZ -
K9U07_RS11190 (2300891) 2300891..2301364 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
K9U07_RS11195 (2301375) 2301375..2302154 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
K9U07_RS11200 (2302144) 2302144..2303022 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
K9U07_RS11205 (2303040) 2303040..2303474 + 435 WP_000948829.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2288434..2298475 10041


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17844.32 Da        Isoelectric Point: 9.6924

>T216691 WP_000347258.1 NZ_CP083512:2298011-2298475 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEILKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEPH

Download         Length: 465 bp

>T216691 NZ_CP113216:4137866-4138084 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12340.91 Da        Isoelectric Point: 4.8616

>AT216691 WP_001604880.1 NZ_CP083512:2297676-2298011 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGLDVNIDDEIGDDE

Download         Length: 336 bp

>AT216691 NZ_CP113216:4137465-4137839 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7B2BI69

Antitoxin

Source ID Structure
AlphaFold DB A0A368IY36

References