Detailed information of TA system TA021669

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 646246..646754
• Replicon: plasmid pRHL1
• Accession: NC_008269
• Organism: Rhodococcus jostii RHA1

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: Q0RYC4
• Locus tag: RHA1_RS38725
• Protein ID: WP_007299764.1
• Coordinates: 646494..646754 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: Q0RYC5
• Locus tag: RHA1_RS38720
• Protein ID: WP_005569061.1
• Coordinates: 646246..646497 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RHA1_RS38700	641679..642173	-	495	WP_007299760.1	SRPBCC family protein	-
RHA1_RS38705	643448..644161	+	714	WP_007299761.1	LppX_LprAFG lipoprotein	-
RHA1_RS38710	644158..645732	+	1575	WP_007299762.1	MFS transporter	-
RHA1_RS38715	645787..646167	-	381	WP_007299763.1	hypothetical protein	-
RHA1_RS38720	646246..646497	+	252	WP_005569061.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
RHA1_RS38725	646494..646754	+	261	WP_007299764.1	Txe/YoeB family addiction module toxin	Toxin
RHA1_RS38735	647420..650395	+	2976	WP_011599396.1	UPF0182 family protein	-
RHA1_RS49075	651022..651229	+	208	Protein_624	alpha/beta hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..1123075	1123075

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 10342.70 Da
• Isoelectric Point: 8.0441

>T21669 WP_007299764.1 NC_008269:646494-646754 [Rhodococcus jostii RHA1]
VKLVWDESAWEDYVWWQAQDRKIVKRINTLLKDVERNGNDGIGKPEPLKDGFQGYWSRRITDEHRLVYKIVDDQIRIAAC
RYHFGH

Nucleotide

• Length: 261 bp

>T21669 NC_008269:646494-646754 [Rhodococcus jostii RHA1]
GTGAAACTTGTCTGGGACGAGTCTGCGTGGGAGGACTACGTCTGGTGGCAGGCGCAGGATCGGAAGATCGTCAAGCGGAT
CAACACATTACTCAAGGATGTCGAACGCAACGGGAACGACGGAATCGGCAAGCCAGAGCCCCTCAAGGACGGTTTTCAGG
GCTACTGGTCCCGGCGTATCACCGATGAGCACCGGCTGGTTTATAAGATTGTCGATGATCAGATCCGCATTGCTGCCTGC
CGTTACCACTTCGGACACTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9358.50 Da
• Isoelectric Point: 4.5216

>AT21669 WP_005569061.1 NC_008269:646246-646497 [Rhodococcus jostii RHA1]
MRTMTYSESRARYAEVLNAVVDDREEIVITRAGHEPVVMVSLDDYESLKETAYLLRSPENARRLLASIDRLESGGGTVRD
LAE

Nucleotide

• Length: 252 bp

>AT21669 NC_008269:646246-646497 [Rhodococcus jostii RHA1]
ATGCGAACCATGACCTACTCGGAGTCTCGCGCGCGGTACGCCGAGGTCCTCAACGCCGTCGTCGATGACCGGGAAGAAAT
CGTCATCACGCGGGCGGGTCACGAGCCGGTGGTGATGGTTTCGCTCGACGACTATGAGTCGTTGAAGGAAACGGCATACT
TGCTCAGGAGTCCGGAGAACGCCCGCCGGCTGTTGGCGTCGATCGATCGGTTGGAGTCCGGCGGGGGAACTGTCCGGGAT
CTGGCTGAGTGA

Structures

Toxin

• AlphaFold DB: Q0RYC4

Antitoxin

• AlphaFold DB: Q0RYC5