Detailed information of TA system TA216669

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 69321..69575
• Replicon: plasmid unnamed1
• Accession: NZ_CP083504
• Organism: Escherichia coli strain elppa5

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: K9U08_RS25500
• Protein ID: WP_001312851.1
• Coordinates: 69321..69470 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	69514..69575 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9U08_RS25470 (64568)	64568..65479	-	912	WP_000440183.1	carbamate kinase	-
K9U08_RS25475 (65490)	65490..66710	-	1221	WP_000410951.1	arginine deiminase	-
K9U08_RS25480 (67417)	67417..68031	+	615	Protein_86	VENN motif pre-toxin domain-containing protein	-
K9U08_RS25485 (68031)	68031..68477	-	447	Protein_87	plasmid replication initiator RepA	-
K9U08_RS25490 (68470)	68470..68544	-	75	WP_032336874.1	RepA leader peptide Tap	-
K9U08_RS25495 (68780)	68780..69037	-	258	WP_000083833.1	replication regulatory protein RepA	-
K9U08_RS25500 (69321)	69321..69470	-	150	WP_001312851.1	Hok/Gef family protein	Toxin
- (69514)	69514..69575	+	62	NuclAT_0	-	Antitoxin
- (69514)	69514..69575	+	62	NuclAT_0	-	Antitoxin
- (69514)	69514..69575	+	62	NuclAT_0	-	Antitoxin
- (69514)	69514..69575	+	62	NuclAT_0	-	Antitoxin
K9U08_RS25505 (69714)	69714..69896	-	183	WP_000968309.1	hypothetical protein	-
K9U08_RS25510 (69997)	69997..70613	+	617	Protein_92	IS1-like element IS1A family transposase	-
K9U08_RS25515 (70651)	70651..72222	-	1572	WP_023149734.1	IS66-like element ISCro1 family transposase	-
K9U08_RS25520 (72242)	72242..72589	-	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
K9U08_RS25525 (72589)	72589..73266	-	678	WP_001339397.1	IS66-like element accessory protein TnpA	-
K9U08_RS25530 (73321)	73321..73410	+	90	Protein_96	IS1 family transposase	-
K9U08_RS25535 (73711)	73711..73923	-	213	WP_005012601.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / aac(3)-IIa	-	1..108485	108485

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T216669 WP_001312851.1 NZ_CP083504:c69470-69321 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T216669 NZ_CP113205:12296-12712 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCGTCCTGCCCTGGGACCGCGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAATGACACGGCCATTGC
CGGGCACGCCATCGCGGCGGGCGCCATCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTGAAATAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT216669 NZ_CP083504:69514-69575 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin