Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 3527751..3528353 Replicon chromosome
Accession NZ_CP083503
Organism Escherichia coli strain elppa5

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag K9U08_RS17330 Protein ID WP_000897305.1
Coordinates 3527751..3528062 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag K9U08_RS17335 Protein ID WP_000356395.1
Coordinates 3528063..3528353 (+) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9U08_RS17305 (3522792) 3522792..3523229 + 438 WP_000560983.1 D-aminoacyl-tRNA deacylase -
K9U08_RS17310 (3523274) 3523274..3524215 + 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
K9U08_RS17315 (3524631) 3524631..3525518 + 888 Protein_3386 hypothetical protein -
K9U08_RS17320 (3525531) 3525531..3526445 + 915 WP_077634137.1 transposase -
K9U08_RS17325 (3526614) 3526614..3527522 - 909 WP_001306651.1 alpha/beta hydrolase -
K9U08_RS17330 (3527751) 3527751..3528062 + 312 WP_000897305.1 hypothetical protein Toxin
K9U08_RS17335 (3528063) 3528063..3528353 + 291 WP_000356395.1 NadS family protein Antitoxin
K9U08_RS17340 (3528712) 3528712..3528990 + 279 WP_001306650.1 hypothetical protein -
K9U08_RS17345 (3529409) 3529409..3529627 + 219 WP_001295676.1 CopG family transcriptional regulator -
K9U08_RS17350 (3529846) 3529846..3530088 + 243 WP_021523315.1 hypothetical protein -
K9U08_RS17355 (3530270) 3530270..3531199 - 930 WP_000027708.1 formate dehydrogenase accessory protein FdhE -
K9U08_RS17360 (3531196) 3531196..3531831 - 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
K9U08_RS17365 (3531828) 3531828..3532730 - 903 WP_000331377.1 formate dehydrogenase O subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T216660 WP_000897305.1 NZ_CP083503:3527751-3528062 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

>T216660 NZ_CP113202:1867502-1867604 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10869.46 Da        Isoelectric Point: 10.8907

>AT216660 WP_000356395.1 NZ_CP083503:3528063-3528353 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp

>AT216660 NZ_CP113202:c1867611-1867466 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References