Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 2678568..2679367 Replicon chromosome
Accession NZ_CP083503
Organism Escherichia coli strain elppa5

Toxin (Protein)


Gene name yhaV Uniprot ID D3GWU5
Locus tag K9U08_RS13270 Protein ID WP_000347272.1
Coordinates 2678903..2679367 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag K9U08_RS13265 Protein ID WP_001307405.1
Coordinates 2678568..2678903 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9U08_RS13250 (2674353) 2674353..2675123 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
K9U08_RS13255 (2675139) 2675139..2676473 - 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
K9U08_RS13260 (2676848) 2676848..2678419 + 1572 WP_001273738.1 galactarate dehydratase -
K9U08_RS13265 (2678568) 2678568..2678903 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
K9U08_RS13270 (2678903) 2678903..2679367 + 465 WP_000347272.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
K9U08_RS13275 (2679422) 2679422..2680231 - 810 WP_000072171.1 aga operon transcriptional regulator AgaR -
K9U08_RS13280 (2680480) 2680480..2681760 + 1281 WP_001521382.1 tagatose-bisphosphate aldolase subunit KbaZ -
K9U08_RS13285 (2681783) 2681783..2682256 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
K9U08_RS13290 (2682267) 2682267..2683046 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
K9U08_RS13295 (2683036) 2683036..2683914 + 879 WP_001298314.1 PTS N-acetylgalactosamine transporter subunit IID -
K9U08_RS13300 (2683932) 2683932..2684366 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17832.26 Da        Isoelectric Point: 9.6924

>T216659 WP_000347272.1 NZ_CP083503:2678903-2679367 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEPH

Download         Length: 465 bp

>T216659 NZ_CP113202:799903-800313 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGTTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT216659 WP_001307405.1 NZ_CP083503:2678568-2678903 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT216659 NZ_CP113202:799656-799922 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829KUD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References