Detailed information of TA system TA216654

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2177759..2178486
• Replicon: chromosome
• Accession: NZ_CP083503
• Organism: Escherichia coli strain elppa5

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A829L2T9
• Locus tag: K9U08_RS10785
• Protein ID: WP_001521139.1
• Coordinates: 2178175..2178486 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K9U08_RS10780
• Protein ID: WP_000126294.1
• Coordinates: 2177759..2178178 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9U08_RS10760 (2172844)	2172844..2173371	-	528	WP_001078777.1	electron transport protein HydN	-
K9U08_RS10765 (2173520)	2173520..2174530	-	1011	WP_015912547.1	DNA-binding transcriptional regulator AscG	-
K9U08_RS10770 (2174790)	2174790..2176247	+	1458	WP_020234027.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
K9U08_RS10775 (2176256)	2176256..2177680	+	1425	WP_020234028.1	6-phospho-beta-glucosidase AscB	-
K9U08_RS10780 (2177759)	2177759..2178178	-	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
K9U08_RS10785 (2178175)	2178175..2178486	-	312	WP_001521139.1	type II toxin-antitoxin system HigB family toxin	Toxin
K9U08_RS10790 (2178653)	2178653..2179123	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
K9U08_RS10795 (2179116)	2179116..2179526	-	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
K9U08_RS10800 (2179523)	2179523..2180290	-	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
K9U08_RS10805 (2180290)	2180290..2180832	-	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
K9U08_RS10810 (2180842)	2180842..2182551	-	1710	WP_001521140.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12493.23 Da
• Isoelectric Point: 9.4783

>T216654 WP_001521139.1 NZ_CP083503:c2178486-2178175 [Escherichia coli]
MHIISKAPFEECARKYPNDALALYSLYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T216654 NZ_CP113198:37437-37853 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCGTCCTGCCCTGGGACCGCGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAATGACACGGCCATTGC
CGGGCACGCCATCGCGGCGGGCGCCATCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTGAAATAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT216654 WP_000126294.1 NZ_CP083503:c2178178-2177759 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT216654 NZ_CP113198:37210-37440 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGATGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: A0A829L2T9