Detailed information of TA system TA216638

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 577765..578136
• Replicon: chromosome
• Accession: NZ_CP083503
• Organism: Escherichia coli strain elppa5

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A0D6ZRD3
• Locus tag: K9U08_RS02930
• Protein ID: WP_001443846.1
• Coordinates: 577987..578136 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	577765..577943 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9U08_RS02905 (573720)	573720..575093	+	1374	WP_000123748.1	ATP-dependent RNA helicase DbpA	-
K9U08_RS02910 (575222)	575222..576157	-	936	WP_001523172.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
K9U08_RS02915 (576209)	576209..577444	-	1236	WP_000040839.1	site-specific integrase	-
K9U08_RS02920 (577446)	577446..577661	-	216	WP_000079604.1	excisionase XisR	-
- (577765)	577765..577943	+	179	NuclAT_0	-	Antitoxin
- (577765)	577765..577943	+	179	NuclAT_0	-	Antitoxin
- (577765)	577765..577943	+	179	NuclAT_0	-	Antitoxin
- (577765)	577765..577943	+	179	NuclAT_0	-	Antitoxin
K9U08_RS02925 (577761)	577761..577949	-	189	WP_001302840.1	DUF1187 family protein	-
K9U08_RS02930 (577987)	577987..578136	-	150	WP_001443846.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
K9U08_RS02935 (578192)	578192..579001	-	810	WP_000166313.1	recombination protein RecT	-
K9U08_RS02940 (578994)	578994..581594	-	2601	WP_000105140.1	exodeoxyribonuclease VIII	-
K9U08_RS02945 (581696)	581696..581971	-	276	WP_001344816.1	hypothetical protein	-
K9U08_RS02950 (582046)	582046..582216	-	171	WP_001352098.1	YdaE family protein	-
K9U08_RS02955 (582216)	582216..582437	-	222	WP_000560223.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	566677..623210	56533

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-48)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5383.12 Da
• Isoelectric Point: 8.3398

>T216638 WP_001443846.1 NZ_CP083503:c578136-577987 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALER

Nucleotide

• Length: 150 bp

>T216638 NZ_CP113192:c4669425-4669141 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCTGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT216638 NZ_CP083503:577765-577943 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A0D6ZRD3

Antitoxin