Detailed information of TA system TA216628

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 30071..30335
• Replicon: plasmid unnamed2
• Accession: NZ_CP083499
• Organism: Escherichia coli strain elppa6

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Z417
• Locus tag: K9U09_RS23605
• Protein ID: WP_001387489.1
• Coordinates: 30183..30335 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	30071..30131 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9U09_RS23580 (25663)	25663..25980	+	318	WP_000118520.1	quaternary ammonium compound efflux SMR transporter SugE	-
K9U09_RS23585 (25977)	25977..26510	-	534	WP_001221666.1	lipocalin family protein	-
K9U09_RS23590 (26604)	26604..27749	-	1146	WP_000976514.1	class C beta-lactamase CMY-2	-
K9U09_RS23595 (28073)	28073..29335	-	1263	WP_228596877.1	IS1380-like element ISEcp1 family transposase	-
K9U09_RS23600 (29488)	29488..29862	-	375	WP_223349261.1	hypothetical protein	-
- (30071)	30071..30131	-	61	NuclAT_0	-	Antitoxin
- (30071)	30071..30131	-	61	NuclAT_0	-	Antitoxin
- (30071)	30071..30131	-	61	NuclAT_0	-	Antitoxin
- (30071)	30071..30131	-	61	NuclAT_0	-	Antitoxin
K9U09_RS23605 (30183)	30183..30335	+	153	WP_001387489.1	Hok/Gef family protein	Toxin
K9U09_RS23610 (30407)	30407..30658	-	252	WP_001291964.1	hypothetical protein	-
K9U09_RS23615 (30958)	30958..31254	+	297	WP_011264046.1	DinQ-like type I toxin DqlB	-
K9U09_RS23620 (31319)	31319..31495	-	177	WP_001054897.1	hypothetical protein	-
K9U09_RS23625 (31888)	31888..32097	+	210	WP_000062603.1	HEAT repeat domain-containing protein	-
K9U09_RS23630 (32169)	32169..32831	-	663	WP_000644796.1	plasmid IncI1-type surface exclusion protein ExcA	-
K9U09_RS23635 (32902)	32902..35070	-	2169	WP_228596879.1	DotA/TraY family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCMY-2 / sul3 / ant(3'')-Ia / floR / lnu(F) / aadA2 / aac(3)-IId	-	1..130873	130873
	-	flank	IS/Tn	blaCMY-2	-	26604..29335	2731

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5761.10 Da
• Isoelectric Point: 8.7948

>T216628 WP_001387489.1 NZ_CP083499:30183-30335 [Escherichia coli]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T216628 NZ_CP113191:c4843327-4842959 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Nucleotide

• Length: 61 bp

>AT216628 NZ_CP083499:c30131-30071 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: U9Z417

Antitoxin