Detailed information of TA system TA216621

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 748..1169
• Replicon: plasmid unnamed1
• Accession: NZ_CP083498
• Organism: Escherichia coli strain elppa6

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: K9U09_RS22680
• Protein ID: WP_096937776.1
• Coordinates: 1044..1169 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	748..946 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9U09_RS22670 (17)	17..779	+	763	Protein_1	plasmid SOS inhibition protein A	-
- (748)	748..946	+	199	NuclAT_0	-	Antitoxin
- (748)	748..946	+	199	NuclAT_0	-	Antitoxin
- (748)	748..946	+	199	NuclAT_0	-	Antitoxin
- (748)	748..946	+	199	NuclAT_0	-	Antitoxin
K9U09_RS22675 (953)	953..1102	+	150	Protein_2	DUF5431 family protein	-
K9U09_RS22680 (1044)	1044..1169	+	126	WP_096937776.1	type I toxin-antitoxin system Hok family toxin	Toxin
K9U09_RS22685 (1470)	1470..1766	-	297	Protein_4	hypothetical protein	-
K9U09_RS22690 (1836)	1836..2042	+	207	WP_000547965.1	hypothetical protein	-
K9U09_RS22695 (2067)	2067..2354	+	288	WP_000107546.1	hypothetical protein	-
K9U09_RS22700 (2473)	2473..3294	+	822	WP_001234475.1	DUF932 domain-containing protein	-
K9U09_RS22705 (3591)	3591..4181	-	591	WP_166494936.1	transglycosylase SLT domain-containing protein	-
K9U09_RS22710 (4533)	4533..4916	+	384	WP_001063020.1	conjugal transfer relaxosome DNA-binding protein TraM	-
K9U09_RS22715 (5108)	5108..5755	+	648	WP_000332520.1	conjugal transfer transcriptional regulator TraJ	-
K9U09_RS22720 (5891)	5891..6106	+	216	WP_001352842.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / mph(A) / sul1 / qacE / aadA5 / sitABCD	senB / iucA / iucB / iucC / iucD / iutA	1..128175	128175

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4806.72 Da
• Isoelectric Point: 8.4890

>T216621 WP_096937776.1 NZ_CP083498:1044-1169 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T216621 NZ_CP113191:508105-508638 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Nucleotide

• Length: 199 bp

>AT216621 NZ_CP083498:748-946 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin