Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3671480..3672279 Replicon chromosome
Accession NZ_CP083497
Organism Escherichia coli strain elppa6

Toxin (Protein)


Gene name yhaV Uniprot ID F4VJD3
Locus tag K9U09_RS17665 Protein ID WP_000347266.1
Coordinates 3671480..3671944 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag K9U09_RS17670 Protein ID WP_001307405.1
Coordinates 3671944..3672279 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9U09_RS17635 (3666481) 3666481..3666915 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
K9U09_RS17640 (3666933) 3666933..3667811 - 879 WP_001315856.1 PTS N-acetylgalactosamine transporter subunit IID -
K9U09_RS17645 (3667801) 3667801..3668580 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
K9U09_RS17650 (3668591) 3668591..3669064 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
K9U09_RS17655 (3669087) 3669087..3670367 - 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
K9U09_RS17660 (3670616) 3670616..3671425 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
K9U09_RS17665 (3671480) 3671480..3671944 - 465 WP_000347266.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
K9U09_RS17670 (3671944) 3671944..3672279 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
K9U09_RS17675 (3672428) 3672428..3673999 - 1572 WP_001273741.1 galactarate dehydratase -
K9U09_RS17680 (3674374) 3674374..3675708 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
K9U09_RS17685 (3675724) 3675724..3676494 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17841.18 Da        Isoelectric Point: 9.4947

>T216617 WP_000347266.1 NZ_CP083497:c3671944-3671480 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRFFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T216617 NZ_CP113188:69391-69540 [Klebsiella pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT216617 WP_001307405.1 NZ_CP083497:c3672279-3671944 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT216617 NZ_CP113188:c69346-69287 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A836NGD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References