Detailed information of TA system TA216592

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 24426..24690
• Replicon: plasmid unnamed4
• Accession: NZ_CP083495
• Organism: Escherichia coli strain elppa8

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Z417
• Locus tag: K9U11_RS25145
• Protein ID: WP_001387489.1
• Coordinates: 24426..24578 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	24630..24690 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9U11_RS25110 (20310)	20310..21538	+	1229	WP_088895425.1	IS3-like element IS2 family transposase	-
K9U11_RS25120 (22495)	22495..22593	+	99	Protein_24	ethanolamine utilization protein EutE	-
K9U11_RS25125 (22665)	22665..22874	-	210	WP_000062603.1	HEAT repeat domain-containing protein	-
K9U11_RS25130 (23266)	23266..23442	+	177	WP_001054897.1	hypothetical protein	-
K9U11_RS25135 (23507)	23507..23803	-	297	WP_011264046.1	DinQ-like type I toxin DqlB	-
K9U11_RS25140 (24103)	24103..24354	+	252	WP_001291964.1	hypothetical protein	-
K9U11_RS25145 (24426)	24426..24578	-	153	WP_001387489.1	Hok/Gef family protein	Toxin
- (24630)	24630..24690	+	61	NuclAT_0	-	Antitoxin
- (24630)	24630..24690	+	61	NuclAT_0	-	Antitoxin
- (24630)	24630..24690	+	61	NuclAT_0	-	Antitoxin
- (24630)	24630..24690	+	61	NuclAT_0	-	Antitoxin
K9U11_RS25150 (24899)	24899..25273	+	375	WP_223349261.1	hypothetical protein	-
K9U11_RS25155 (25426)	25426..26583	+	1158	Protein_31	IS1380-like element ISEcp1 family transposase	-
K9U11_RS25160 (26639)	26639..27336	+	698	WP_095033700.1	IS1-like element IS1B family transposase	-
K9U11_RS25165 (27347)	27347..27655	-	309	WP_039023235.1	transcription termination/antitermination NusG family protein	-
K9U11_RS25170 (28097)	28097..28384	-	288	WP_000074855.1	conjugal transfer protein TraA	-
K9U11_RS25175 (28422)	28422..28580	-	159	Protein_35	ash family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCMY-42 / blaCTX-M-14	-	1..63040	63040
	-	inside	IScluster/Tn	blaCMY-42	-	18809..26914	8105

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5761.10 Da
• Isoelectric Point: 8.7948

>T216592 WP_001387489.1 NZ_CP083495:c24578-24426 [Escherichia coli]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T216592 NZ_CP113173:c4750896-4750612 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGTGAATAAACGGCTCTAA

Antitoxin

Nucleotide

• Length: 61 bp

>AT216592 NZ_CP083495:24630-24690 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: U9Z417

Antitoxin