Detailed information of TA system TA216591

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 133207..133864
• Replicon: plasmid unnamed3
• Accession: NZ_CP083494
• Organism: Escherichia coli strain elppa8

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: K9U11_RS24960
• Protein ID: WP_000270043.1
• Coordinates: 133514..133864 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K9U11_RS24955
• Protein ID: WP_000124640.1
• Coordinates: 133207..133509 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9U11_RS24905 (K9U11_24925)	128832..129260	+	429	WP_000591074.1	hypothetical protein	-
K9U11_RS24910 (K9U11_24930)	129317..129676	+	360	WP_000422768.1	hypothetical protein	-
K9U11_RS24915 (K9U11_24935)	129676..130122	+	447	WP_228596105.1	Fe3+-siderophore ABC transporter permease	-
K9U11_RS24920 (K9U11_24940)	130119..130637	+	519	WP_228596106.1	nitrite reductase	-
K9U11_RS24925 (K9U11_24945)	130637..130867	+	231	WP_000972663.1	hypothetical protein	-
K9U11_RS24930 (K9U11_24950)	130854..131711	+	858	WP_001167032.1	hypothetical protein	-
K9U11_RS24935 (K9U11_24955)	131737..131925	+	189	WP_001270411.1	hypothetical protein	-
K9U11_RS24940 (K9U11_24960)	131942..132469	+	528	WP_001236377.1	thermonuclease family protein	-
K9U11_RS24945 (K9U11_24965)	132527..132799	+	273	WP_228596109.1	HU family DNA-binding protein	-
K9U11_RS24950 (K9U11_24970)	132887..133180	+	294	WP_001239997.1	chromosome segregation protein ParM	-
K9U11_RS24955 (K9U11_24975)	133207..133509	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
K9U11_RS24960 (K9U11_24980)	133514..133864	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K9U11_RS24965 (K9U11_24985)	134027..134575	+	549	WP_001061574.1	transcriptional regulator	-
K9U11_RS24970 (K9U11_24990)	134916..135110	+	195	WP_000343597.1	hypothetical protein	-
K9U11_RS24975 (K9U11_24995)	135121..135492	+	372	WP_000516916.1	hypothetical protein	-
K9U11_RS24980 (K9U11_25000)	135485..135955	+	471	WP_001281821.1	hypothetical protein	-
K9U11_RS24985 (K9U11_25005)	135970..136305	-	336	WP_000683476.1	hypothetical protein	-
K9U11_RS24990 (K9U11_25010)	136402..136890	+	489	WP_228596111.1	DUF1643 domain-containing protein	-
K9U11_RS24995 (K9U11_25015)	136893..137390	+	498	WP_228596113.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(3)-IId / floR / tet(A) / aph(3')-Ia / aph(6)-Id / aph(3'')-Ib / sul2	-	1..137552	137552

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T216591 WP_000270043.1 NZ_CP083494:c133864-133514 [Escherichia coli]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T216591 NZ_CP113173:c4665917-4665576 [Klebsiella pneumoniae]
ATGAATACTTTACCTGTAATAAACCAGCGGGCTATTCAGTTATGCCCATCGCCTGTAACTATCTGGCAAACGTTGCTAAC
TCGTTTACTGGAACAACACTACGGGCTGGCGCTGAACGACACACCGTTCGGTGATGAAAGCGTGATTCAGGAACATATCG
AGGCCGGTATTACACTCGTTGATGCCGTTAACTTCTTGGTGGAAAAGTACGAGCTGGTTCGTATCGACCGCTGGGCGTTT
GGCTGGTTGGAACCATCTCCATACTTGCGAGCTGAAGACATTCTGCGAATGCGTCGGGACATGGGGCTGTTGCGAGGCTG
CAACCACACTGCAGCAATGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT216591 WP_000124640.1 NZ_CP083494:c133509-133207 [Escherichia coli]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT216591 NZ_CP113173:c4666302-4665952 [Klebsiella pneumoniae]
ATGTCGAATAAGACACAGACAGTGAATGGTGATACTGCCGAGCCCAGGTGGGGCTTGAGTTGTAACGTCATCCCTTGTTT
TGGTGCACGTCTGGTCCAGGAAGGTAACCGCCTACATTATCTGGCTGACCGCGCCAGCATAACAGGTCAGTTCAATGAAG
CGGATTTAATCCATCCTGACCAGGCATTCCCGGTCCTGTTGAAGCAGGCAGAGCTCATGCTTACCAGCGGCGAACTGAAT
CCCCGACACCAGCATTGCGTCACGTTCTGTGAAAAGGGGTTGACCTGCGAAGCTGATACGCTGGGTTCCTGTGGCCACGT
CTATATTGTCATTTACCCTACTCAACGCTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9