Detailed information of TA system TA216588

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 51666..51920
• Replicon: plasmid unnamed2
• Accession: NZ_CP083493
• Organism: Escherichia coli strain elppa8

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: K9U11_RS23905
• Protein ID: WP_001312851.1
• Coordinates: 51771..51920 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	51666..51727 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9U11_RS23880 (48414)	48414..49160	+	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-
K9U11_RS23885 (49219)	49219..50079	+	861	WP_000704514.1	alpha/beta hydrolase	-
K9U11_RS23890 (50182)	50182..50742	+	561	WP_000139315.1	fertility inhibition protein FinO	-
K9U11_RS23895 (50878)	50878..51090	+	213	WP_001299730.1	ANR family transcriptional regulator	-
K9U11_RS23900 (51336)	51336..51410	+	75	Protein_64	endonuclease	-
- (51666)	51666..51727	-	62	NuclAT_1	-	Antitoxin
- (51666)	51666..51727	-	62	NuclAT_1	-	Antitoxin
- (51666)	51666..51727	-	62	NuclAT_1	-	Antitoxin
- (51666)	51666..51727	-	62	NuclAT_1	-	Antitoxin
K9U11_RS23905 (51771)	51771..51920	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
K9U11_RS23910 (52204)	52204..52461	+	258	WP_000083845.1	replication regulatory protein RepA	-
K9U11_RS23915 (52478)	52478..52729	-	252	WP_228596070.1	replication protein RepA	-
K9U11_RS23920 (52720)	52720..52767	+	48	WP_229471593.1	hypothetical protein	-
K9U11_RS23925 (52760)	52760..53242	+	483	WP_001273588.1	hypothetical protein	-
K9U11_RS23930 (53235)	53235..54092	+	858	WP_228596032.1	incFII family plasmid replication initiator RepA	-
K9U11_RS23935 (54793)	54793..54933	+	141	WP_001333237.1	hypothetical protein	-
K9U11_RS23940 (55031)	55031..55684	+	654	WP_228596034.1	CPBP family intramembrane metalloprotease	-
K9U11_RS23945 (55777)	55777..56034	+	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
K9U11_RS23950 (55967)	55967..56368	+	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / sitABCD	iucB / iucC / iucD / iutA / iucA	1..96208	96208

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T216588 WP_001312851.1 NZ_CP083493:51771-51920 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T216588 NZ_CP113173:c2708941-2707619 [Klebsiella pneumoniae]
ATGGCGACGCTTACCACCTGGATGAACAATGTGCGCGTGGGAACCTTAACCCGCCAGGCTAACGGGGCGCACAGTTTTCG
CTATGACGAAGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCACTGCCGCTGCAGTATGGCAATATCACTG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGACAGCCCGCAGGTCCGCGACCGGATCGTCAGGCGCTATCAG
GCGCGCTCGAAGCAGCCGTTCGATCTGCTGGCCGAAGTCGGGCGGGATAGCGTAGGGGCGGTGACCCTCCTGCCGCCGGG
GGAAGAGGCGCACCTGGAAGGATTACGCTGGCAGACGCTGGACGAGGCGCAGCTCACCACTTTGCTGACCGCTTACCAGT
CGGATATCCCCCTCGGGATGATTACCAGCCAGGACGATTTCCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGAGCAGTGGTGTATTCCGCAGGGCGCCACGCCGACCACGCATATCATTAAGCTGCCCATCGGCGAGAT
AAAGCAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAATGAGTATCTGTGCCTGGCGCTGGCCAGAGAACTGG
GCCTGGCGGTGCCGGAAGCGGAGATCATCGCCACTCCGCGGATCCGCGCCCTGGCGGTAACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGACGGGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCTTTTGGTCTTCCTTCAGCGATGAAATA
TGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCGAGTGAAGCGCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTCTTCCAGTGGCTGACGGGGGCGACGGACGGTCACGCAAAAAACTTCTCAATTTACCTGTTG
CCCGGCGGCAGCTACCGCTTAACACCGTTCTACGACATTATTTCGGCGTTTCCGGTGCTGGGCGGCACGGGACTGCATCT
GCGCGATCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGTAAAACAGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCGACGGCCAAAGCGGTGAACTTTCCCCGGGAGCAGATGCTGGCGATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGCTCGCCGGACGCTGCCGGCGGACTTTTCCAGCCACGTCTGGCAGGCCATTACAGAGAACATGCT
GAAGCTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGCTATAG

Antitoxin

Nucleotide

• Length: 62 bp

>AT216588 NZ_CP083493:c51727-51666 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin