Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4298680..4299407 Replicon chromosome
Accession NZ_CP083492
Organism Escherichia coli strain elppa8

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag K9U11_RS20780 Protein ID WP_000550189.1
Coordinates 4298680..4298994 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag K9U11_RS20785 Protein ID WP_000560266.1
Coordinates 4298991..4299407 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9U11_RS20760 (4294838) 4294838..4295824 - 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -
K9U11_RS20765 (4295903) 4295903..4296595 - 693 WP_000942548.1 vancomycin high temperature exclusion protein -
K9U11_RS20770 (4296672) 4296672..4297175 - 504 WP_001333820.1 M48 family metallopeptidase -
K9U11_RS20775 (4297260) 4297260..4298396 + 1137 WP_228595433.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
K9U11_RS20780 (4298680) 4298680..4298994 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
K9U11_RS20785 (4298991) 4298991..4299407 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
K9U11_RS20790 (4299452) 4299452..4301470 - 2019 WP_000121435.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
K9U11_RS20795 (4301896) 4301896..4304247 - 2352 WP_228595434.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T216579 WP_000550189.1 NZ_CP083492:4298680-4298994 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T216579 NZ_CP113168:c4839111-4838236 [Klebsiella pneumoniae]
ATGCAGGTTTTACCCTATGTTGCTGTCGAACGGGAATTTGCGCTGAAAGGTGGCACTGCCATCAATCTCTTTATCCGCGA
TTTCCCTCGATTATCGGTGGATATCGACCTCGCCTGGGTGCCCCTGGAAAGCAGAGCCATCGCTTTACCTCATATACGGG
ATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAGGCAGGTATGAGCGCTGTATTACAAGCTAATCGTTCAGATGAG
ATGCGTGTCATTGTTACTACCGATAGCGCGCAAATAAAGATAGAGGTTTCACCGGTCGCCCGGGGAACATTATATCCACC
ACAGGAGCGAGAAGTGGTGGGGGCGGTAGAGGACGTTTTTGGCTATGCCAGCCTTCCAGTGGTTTCGTTGCCTGACCTCT
ACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCATCCCAGAGATTTTTATGACGTGAAATTACTTCTTGATGCGCAG
GAGCTTGACCGTCCCATTTTCAATGGGTTTATCGTGTATTTACTGAGCCATAATCGGCCTCTGGCTGAAGTGCTGAATCC
TCGCTGGAAAGATATTGCTGAACCTTTTTACCGTGAATTCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGA
CAGCAGTCCCCAACAGGATGATTGCTGCGTTAAAATCCTGTATCACACAACAGGATGTCGATTTTTTACTGTCATTTAAA
CGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAATGCGTATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATAT
TCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAGATAAACTGGAAAAGGTGCTGGCCGAGTGGCGCAGTTAG

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT216579 WP_000560266.1 NZ_CP083492:4298991-4299407 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT216579 NZ_CP113168:c4839917-4839159 [Klebsiella pneumoniae]
ATGGCAACAAAATTAAACTGGCTGCTGCAAAACAGCGCTCCCGGAGATGTGATCCTTCAGTCCTGGTTAAGCCAGCATGC
CATCTCTCCCTCTCTGGCCTTTAAATACGCGCAAAGTGGCTGGTTAAAAAAAAGAGGCAACGGCGTATATGCGCGTGCGG
GACGTGAGCCGGAGTGGAACGATGCATTAGCATGTTTGCAAAATCAGCTTGCCGCTCCGGTGTATGTTGCGGGGCTCAGT
AGCTTAGTCTGGCAGGGCCGTTCACACTATCTACAGCTAAAACAAAACCAGTGCTGGCTTAGTGTGGAAAATAAAGCGCT
GCTGCCGAAATGGTTCAGGGAATTCCCGGGTGTGGAGTGGATAGTCATATCGGGGAAAAAACTGCCGGTGCTGGATGATA
GATACCGTGTAACGCTTGATATAAAAGGGAAAAAACTCATCGGCAGTGCGCCAGAACTGGCTGCTTACGAGTTGCTGAGC
GCGGTGCCGGGGACCCTCTCATTTAACCACGCTGCAGAGCTTTTCCAGGGGCTGGTTAATCTTAATCCTCGTAAAGTAGA
GTATCTTCTTTCCGTCAGCCAGTCCGTGCAGGCCAAGCGTTTGTATCTCTTCTTCGCCAGTTTTTACGAGCATGGCTGGC
TCAAACGAATCGATAGCCAAAAAATCGATCTGGGAGCGGGCAAGCGGCAAATCGTGGAAAATGGAAAGTTCAATGCTCAA
TACCAAATCACAGTGCCTGAACGCTTTCAAAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References