Detailed information of TA system TA216548

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 42252..42505
• Replicon: plasmid unnamed3
• Accession: NZ_CP083484
• Organism: Escherichia coli strain elppa9

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: K9U12_RS24820
• Protein ID: WP_001312851.1
• Coordinates: 42356..42505 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	42252..42311 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9U12_RS24785 (38029)	38029..38889	+	861	WP_000704523.1	alpha/beta hydrolase	-
K9U12_RS24790 (38992)	38992..39552	+	561	WP_000139321.1	fertility inhibition protein FinO	-
K9U12_RS24795 (39681)	39681..39892	+	212	Protein_48	ANR family transcriptional regulator	-
K9U12_RS24800 (40137)	40137..40598	+	462	WP_001233838.1	thermonuclease family protein	-
K9U12_RS24805 (40644)	40644..40853	+	210	WP_001298565.1	hemolysin expression modulator Hha	-
K9U12_RS24810 (40891)	40891..41481	+	591	WP_033807967.1	DUF2726 domain-containing protein	-
K9U12_RS24815 (41636)	41636..42109	+	474	WP_016240489.1	hypothetical protein	-
- (42252)	42252..42311	-	60	NuclAT_1	-	Antitoxin
- (42252)	42252..42311	-	60	NuclAT_1	-	Antitoxin
- (42252)	42252..42311	-	60	NuclAT_1	-	Antitoxin
- (42252)	42252..42311	-	60	NuclAT_1	-	Antitoxin
K9U12_RS24820 (42356)	42356..42505	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
K9U12_RS24825 (42790)	42790..43038	+	249	WP_000083839.1	replication regulatory protein RepA	-
K9U12_RS24830 (43283)	43283..43357	+	75	WP_001365705.1	RepA leader peptide Tap	-
K9U12_RS24835 (43350)	43350..44228	+	879	WP_228571354.1	incFII family plasmid replication initiator RepA	-
K9U12_RS24840 (44910)	44910..45050	+	141	WP_001333237.1	hypothetical protein	-
K9U12_RS24845 (45641)	45641..45802	+	162	WP_228571357.1	hypothetical protein	-
K9U12_RS24850 (45895)	45895..46152	+	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
K9U12_RS24855 (46085)	46085..46486	+	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
K9U12_RS24860 (46735)	46735..47150	+	416	Protein_61	IS1-like element IS1B family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(3)-IId	rmpA2 / iutA / iucD / iucC / iucB / iucA / rmpA / iroN / iroD / iroC / iroB	1..224105	224105

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T216548 WP_001312851.1 NZ_CP083484:42356-42505 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T216548 NZ_CP113163:c3419579-3419205 [Citrobacter braakii]
ATGGTCACAGGATCTGCGCTTTTTGATACCAACATTCTCATCGACTTGTTTAGCGGGCGAAGTGAAGCAAAACTAGCGAT
AGAAACATGGCCGCCGCAGAACGCAATCAGCCTCATTACATGGATGGAGTTAATGGTGGGCGCAAAAAAATATCATCAGG
AATCCCGCACACGCGTTGCGCTAGGCGCTTTTAATGTGATCGGTGTTTCACAGGATATTGCTGAACGAAGCGTGAGTATC
AGGCAGGAATATGGGATGAAATTGCCGGATGCCATCATTTTGGCCACTGCGCAGATTCATCGTCTTACACTGGTTACGCG
TAATACCAGGGATTTTGCAGGCATATCCGGCGTGGTCACACCGTATACGTTATAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT216548 NZ_CP083484:c42311-42252 [Escherichia coli]
AATGTACTTCATGCAGACCTCACGAGTTAATGGATTAACAAGTGGGGTCTTTGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin