Detailed information of TA system TA216545

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 836..1105
• Replicon: plasmid unnamed3
• Accession: NZ_CP083484
• Organism: Escherichia coli strain elppa9

Toxin (Protein)

• Gene name: hok
• Uniprot ID: G9G195
• Locus tag: K9U12_RS24570
• Protein ID: WP_001323520.1
• Coordinates: 989..1105 (+)
• Length: 39 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	836..901 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9U12_RS24555	1..710	+	710	Protein_0	plasmid SOS inhibition protein A	-
-	679..903	+	225	NuclAT_0	-	-
-	679..903	+	225	NuclAT_0	-	-
-	679..903	+	225	NuclAT_0	-	-
-	679..903	+	225	NuclAT_0	-	-
K9U12_RS24560	688..867	-	180	WP_001309233.1	hypothetical protein	-
-	836..901	-	66	-	-	Antitoxin
K9U12_RS24565	889..1038	+	150	Protein_2	plasmid maintenance protein Mok	-
K9U12_RS24570	989..1105	+	117	WP_001323520.1	type I toxin-antitoxin system Hok family toxin	Toxin
K9U12_RS24575	1422..1718	-	297	Protein_4	hypothetical protein	-
K9U12_RS24580	2019..2315	+	297	WP_001272251.1	hypothetical protein	-
K9U12_RS24585	2426..3247	+	822	WP_001234445.1	DUF932 domain-containing protein	-
K9U12_RS24590	3544..4134	-	591	WP_000252683.1	transglycosylase SLT domain-containing protein	-
K9U12_RS24595	4469..4852	+	384	WP_001354030.1	conjugal transfer relaxosome DNA-binding protein TraM	-
K9U12_RS24600	5046..5717	+	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
K9U12_RS24605	5854..6081	+	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(3)-IId	rmpA2 / iutA / iucD / iucC / iucB / iucA / rmpA / iroN / iroD / iroC / iroB	1..224105	224105

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (1-34)

Sequences

Toxin

Protein

• Length: 39 a.a.
• Molecular weight: 4455.23 Da
• Isoelectric Point: 8.5110

>T216545 WP_001323520.1 NZ_CP083484:989-1105 [Escherichia coli]
VCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 117 bp

>T216545 NZ_CP113163:c1653601-1653269 [Citrobacter braakii]
ATGGACAGAGGGGAAATCTGGCTCGTCTCACTGGATCCTACAGCCGGACATGAACAAAGCGGAAAACGTCCGGTACTTAT
CGTTTCTCCGGCCTCATTTAATAAGTTCACCCGGCTACCCGTTGTTGTTCCCATCACCAGCGGCGGAAACTTCGCCCGCA
CAGCCGGTTTTACCGTCTCCCTGGACGGCGCGGGAACAAAAACAACGGGTGTTATTCGCTGTGACCAGCCCAGAACGATT
GATATGGGTGCCCGGAATGGTAAGCGTCTGGAACGTATACCCGAAGCTGTCGTAAATGAAGTGCTGGCCCGACTGGAAGC
CATTCTAAGCTGA

Antitoxin

Nucleotide

• Length: 66 bp

>AT216545 NZ_CP083484:c901-836 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Toxin

• AlphaFold DB: G9G195

Antitoxin