Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4392682..4393409 Replicon chromosome
Accession NZ_CP083481
Organism Escherichia coli strain elppa9

Toxin (Protein)


Gene name higB Uniprot ID A0A829L2T9
Locus tag K9U12_RS21290 Protein ID WP_001521139.1
Coordinates 4392682..4392993 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag K9U12_RS21295 Protein ID WP_000126294.1
Coordinates 4392990..4393409 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9U12_RS21265 (4388617) 4388617..4390326 + 1710 WP_001521140.1 formate hydrogenlyase subunit HycE -
K9U12_RS21270 (4390336) 4390336..4390878 + 543 WP_000493785.1 formate hydrogenlyase subunit HycF -
K9U12_RS21275 (4390878) 4390878..4391645 + 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
K9U12_RS21280 (4391642) 4391642..4392052 + 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
K9U12_RS21285 (4392045) 4392045..4392515 + 471 WP_000132961.1 hydrogenase maturation peptidase HycI -
K9U12_RS21290 (4392682) 4392682..4392993 + 312 WP_001521139.1 type II toxin-antitoxin system HigB family toxin Toxin
K9U12_RS21295 (4392990) 4392990..4393409 + 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
K9U12_RS21300 (4393488) 4393488..4394912 - 1425 WP_020234028.1 6-phospho-beta-glucosidase AscB -
K9U12_RS21305 (4394921) 4394921..4396378 - 1458 WP_020234027.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
K9U12_RS21310 (4396638) 4396638..4397648 + 1011 WP_015912547.1 DNA-binding transcriptional regulator AscG -
K9U12_RS21315 (4397797) 4397797..4398324 + 528 WP_001078777.1 electron transport protein HydN -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12493.23 Da        Isoelectric Point: 9.4783

>T216544 WP_001521139.1 NZ_CP083481:4392682-4392993 [Escherichia coli]
MHIISKAPFEECARKYPNDALALYSLYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

>T216544 NZ_CP113163:c1531411-1531308 [Citrobacter braakii]
GGCAGGGTAACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT216544 WP_000126294.1 NZ_CP083481:4392990-4393409 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT216544 NZ_CP113163:1531303-1531446 [Citrobacter braakii]
CACATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTT
GGCATTAATGCAGGCTAAGTTACCCTGCCATTTAAGAATAGATGACAGCGCCAGGTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829L2T9

Antitoxin

Source ID Structure

References