Detailed information of TA system TA216539

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3112003..3112605
• Replicon: chromosome
• Accession: NZ_CP083481
• Organism: Escherichia coli strain elppa9

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: K9U12_RS15205
• Protein ID: WP_000897305.1
• Coordinates: 3112294..3112605 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K9U12_RS15200
• Protein ID: WP_000356395.1
• Coordinates: 3112003..3112293 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9U12_RS15165 (3107626)	3107626..3108528	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
K9U12_RS15170 (3108525)	3108525..3109160	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
K9U12_RS15175 (3109157)	3109157..3110086	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
K9U12_RS15180 (3110268)	3110268..3110510	-	243	WP_021523315.1	hypothetical protein	-
K9U12_RS15185 (3110729)	3110729..3110947	-	219	WP_001295676.1	CopG family transcriptional regulator	-
K9U12_RS15190 (3111366)	3111366..3111644	-	279	WP_001306650.1	hypothetical protein	-
K9U12_RS15195 (3111706)	3111706..3111918	-	213	WP_000197769.1	hypothetical protein	-
K9U12_RS15200 (3112003)	3112003..3112293	-	291	WP_000356395.1	NadS family protein	Antitoxin
K9U12_RS15205 (3112294)	3112294..3112605	-	312	WP_000897305.1	hypothetical protein	Toxin
K9U12_RS15210 (3112834)	3112834..3113742	+	909	WP_001306651.1	alpha/beta hydrolase	-
K9U12_RS15215 (3113911)	3113911..3114825	-	915	WP_077634137.1	transposase	-
K9U12_RS15220 (3114838)	3114838..3115725	-	888	Protein_2977	hypothetical protein	-
K9U12_RS15225 (3116141)	3116141..3117082	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
K9U12_RS15230 (3117127)	3117127..3117564	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T216539 WP_000897305.1 NZ_CP083481:c3112605-3112294 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T216539 NZ_CP113115:c1661889-1661635 [Streptococcus pneumoniae]
TTGTATAAATTAGTTCCAACAAGACGTTTTATCAAGCAATTGAAAAAATTGGACCGTTATACGCAGAAGCTAATTACAAA
CTATTTACAAACCAATGTTTTGGAAGACCCAAGACGACACGGAAAGGCTTTGGTTGGTAATCGCGTTGGTCAATGGCGCT
ATAGAATTGGTAATTATCGAGTTATCGTACAAATTGTAGATGATGAATTAGTTGTTGCTACTCTAGAAGTTGGTCATCGG
AGAGATATTTATTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT216539 WP_000356395.1 NZ_CP083481:c3112293-3112003 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT216539 NZ_CP113115:c1662133-1661891 [Streptococcus pneumoniae]
ATGACTACTATTACATTAAAAGTTTCTGAAGCTGATAAAACATTTATGAAAGCAATGGCTAAGTTTGAAGGAGTTTCCCT
TTCGGAACTTATTCGCACCAAAACTCTTGAAGCCCTAGAAGATGAATACGATGCTCGTGTGGCAGATTTAGCCTATCAAG
AGTATTTAGAAGACTTGGAAAAAGGAGTTGAACCCATTACTTGGGAAGAAATGATGCATGATTTAGGCTTGAAGGATGAA
TAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7