Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 2153221..2153915 Replicon chromosome
Accession NZ_CP083481
Organism Escherichia coli strain elppa9

Toxin (Protein)


Gene name yafO Uniprot ID A0A0D8WHS4
Locus tag K9U12_RS10630 Protein ID WP_001521903.1
Coordinates 2153221..2153619 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag K9U12_RS10635 Protein ID WP_000554758.1
Coordinates 2153622..2153915 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (2149051) 2149051..2149131 - 81 NuclAT_11 - -
- (2149051) 2149051..2149131 - 81 NuclAT_11 - -
- (2149051) 2149051..2149131 - 81 NuclAT_11 - -
- (2149051) 2149051..2149131 - 81 NuclAT_11 - -
K9U12_RS10600 (2148391) 2148391..2149635 - 1245 WP_000189541.1 esterase FrsA -
K9U12_RS10605 (2149727) 2149727..2150185 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
K9U12_RS10610 (2150446) 2150446..2151903 + 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
K9U12_RS10615 (2151960) 2151960..2152312 - 353 Protein_2080 peptide chain release factor H -
K9U12_RS10620 (2152308) 2152308..2152514 - 207 Protein_2081 RtcB family protein -
K9U12_RS10625 (2152759) 2152759..2153211 - 453 WP_023144376.1 GNAT family N-acetyltransferase -
K9U12_RS10630 (2153221) 2153221..2153619 - 399 WP_001521903.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
K9U12_RS10635 (2153622) 2153622..2153915 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
K9U12_RS10640 (2153967) 2153967..2155022 - 1056 WP_001226177.1 DNA polymerase IV -
K9U12_RS10645 (2155093) 2155093..2155878 - 786 WP_000207556.1 putative lateral flagellar export/assembly protein LafU -
K9U12_RS10650 (2155850) 2155850..2157562 + 1713 Protein_2087 flagellar biosynthesis protein FlhA -
K9U12_RS10655 (2157641) 2157641..2157994 + 354 WP_024166466.1 type II toxin-antitoxin system HicB family antitoxin -
K9U12_RS10660 (2158051) 2158051..2158800 - 750 WP_016233951.1 C40 family peptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2143158..2153915 10757


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15471.88 Da        Isoelectric Point: 8.0949

>T216533 WP_001521903.1 NZ_CP083481:c2153619-2153221 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGILPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T216533 NZ_CP113106:2646609-2647187 [Pseudomonas aeruginosa]
ATGTCCCTCGAACTGCTGAGCAACCGCCTCGAATTCCTCCGCGAAGCCGAACGCCTGAAAGACGTCCTGCGCTCCGCGCA
CACCTCCTCCGGCCGCCCGGAAAGCACCGCCGAACATAGCTGGCGGCTGTGCCTGATGGCACTGGCCTTCGAGGACCAAC
TGGCCGGCCTGGACCTCGGCAAGGTCCTGCGCATGTGCGTGGTCCACGACCTCGGCGAAGCGATCCACGGCGACATCCCC
GCCGTCGAGCAGGCCGCGCACCCGGACAAAGGCGAACAGGAACGCGCCGACCTGTTGCAGCTGACCCGTCACCTCGACGC
GCCGCTGCGCGACCGCCTGCTCGCCCTGTGGGACGAATACGAGCGCGGCGAGTCCGCCGAAGCCCTGGCAGTGAAGGCCC
TGGACAAGCTGGAAACCCTGCTCCAGCACACCCAGGGCGACAATCCGGCGGACTTCGACTATGCCTTCAACCTCGACTAC
GGCCGTCGCTATACCGACCGCGCACCGCTGTTCAAGACCCTGCGCGAGCTACTCGACGCCAGGACCCGACAGCGCCTCGC
AGTTCGGCGCGACGCCTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT216533 WP_000554758.1 NZ_CP083481:c2153915-2153622 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT216533 NZ_CP113106:2645966-2646520 [Pseudomonas aeruginosa]
ATGACCGAGCCTCTCCCTTCTTCGTCCCTGGGCCCCGCCCTGCGTCGCTGGCGCCTGCTGCACCGGGTCAAGCAAACGCA
TGCCGCCGAATTGCTCGGCGTGGCGCAATCGACCATCTCGCGCTGGGAGTCCGGTACCCAGGCGCTGCTGGGGGAGGAAC
GGGCACGCCTGGAGCGTCTGCTCGGCGCCCGCCTGGAAGCCGCCGCCGACCAGGCACTGGCCCGCCTGGTCGAGGCCAAC
CCGCAACCCGTGCACCTGATCTGCGACCTCACCCATCGCCTGTTGGCCTGTTCGCCGGCACGCGCCGCGCAATTCGGCGT
GCCTCTCGGCGAACTGCTCGACCGTTCGCTGTGGCCCTATTGCAGCGAGGAAATCCTCCGCCAGGAAGCCACCCTCGAGG
AACTCGGCTGGCGCGAACTGCTGGCACCGCCGGCGCTGGAGTTCGCCAGCGGAGCGAACGCCTCGGCCATAGTGCCGATC
CGGCGCAGCCGTTGCCGCTGGACCCGCATGACCCTCTCGGACGGACGCGCGGTGCGCCTGGTGGAAACCCTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0D8WHS4

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References