Detailed information of TA system TA216514

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 145572..145826
• Replicon: plasmid unnamed1
• Accession: NZ_CP083479
• Organism: Escherichia coli strain elppa10

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: K9U13_RS23990
• Protein ID: WP_001312851.1
• Coordinates: 145677..145826 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	145572..145633 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9U13_RS23965 (142320)	142320..143066	+	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-
K9U13_RS23970 (143125)	143125..143985	+	861	WP_000704514.1	alpha/beta hydrolase	-
K9U13_RS23975 (144088)	144088..144648	+	561	WP_000139315.1	fertility inhibition protein FinO	-
K9U13_RS23980 (144784)	144784..144996	+	213	WP_001299730.1	ANR family transcriptional regulator	-
K9U13_RS23985 (145242)	145242..145316	+	75	Protein_161	endonuclease	-
- (145572)	145572..145633	-	62	NuclAT_1	-	Antitoxin
- (145572)	145572..145633	-	62	NuclAT_1	-	Antitoxin
- (145572)	145572..145633	-	62	NuclAT_1	-	Antitoxin
- (145572)	145572..145633	-	62	NuclAT_1	-	Antitoxin
K9U13_RS23990 (145677)	145677..145826	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
K9U13_RS23995 (146110)	146110..146367	+	258	WP_000083845.1	replication regulatory protein RepA	-
K9U13_RS24000 (146384)	146384..146635	-	252	WP_223195197.1	replication protein RepA	-
K9U13_RS24005 (146626)	146626..146673	+	48	WP_229471593.1	hypothetical protein	-
K9U13_RS24010 (146666)	146666..147148	+	483	WP_001273588.1	hypothetical protein	-
K9U13_RS24015 (147141)	147141..147998	+	858	WP_000774297.1	incFII family plasmid replication initiator RepA	-
K9U13_RS24020 (148937)	148937..149590	+	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(3)-IId / blaTEM-1A / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / floR / dfrA14 / sul3 / aph(3')-Ia / sitABCD	iroE / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA	1..149801	149801

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T216514 WP_001312851.1 NZ_CP083479:145677-145826 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T216514 NZ_CP113096:c6841766-6840996 [Pseudomonas citronellolis]
ATGGCCCTCGACGCCGGCGCCGAACTGTGGCGCGAATGCCGGGGCGAGCAGGTCATAGCCCCGCTGCGGGGCAAGCTGGT
GCGCCTGGTGGAGAGCCAGGAGCAGGTCGCGACCCTGCAACTGGTGGATACCCTGGAGGAGCAGGCCCTGCTGGAAGAGC
TGATCGAGACCAGCAAGCCGCACCTGCCGCCGTCCACCGAAACCCTGCACTACCTGCTCAAGACGCCGTTCCGCTACCCG
CCGCTGCGCTGGGGCTCGCGCTTCGGCCGCCGCCACGAACCCAGCCTGTTCTACGCCGCGTTGCGCCTGGAGACGGCCAT
GGCCGAGGCGGCCTATTACCGCTTCGTGCTCTGGGAGGGGATGGCCAGCCCGCCGCCCAGCGGGCGCATCCTTTCCGAGC
ACGCTTCTTTCGAGGCGCGTTTCCAGGTGCAACGGGGCATACGCCTGCAGGCGCCGCCCTTCGACAGCTATGCCGAGCGC
CTGCAGGACCCGCGGGACTACCGCCTGACCCAGGCGCTGGGCAGCGCCATGCGCGCTGCCGCGGTGGAGGCCTTCGAATA
CCGCTCGGCGCGCTGCCCGGAAGGTGGGCTGAATGCCGCGCTCTTCGTGCCTTCGGCCTTCGTCGACAAGCGCCCGCGCA
ACCTCATGCCCTGGCTGTGCGAGACCACCGCCGACTACGTGGCCTTCAAGCATGCCCAGGTGCCGGGGGCGCCGCGGCTG
TTCCCGCGCGCGGGATTCCTGGTCGAGGGCGACCTGCCGCATCCGGCGTGA

Antitoxin

Nucleotide

• Length: 62 bp

>AT216514 NZ_CP083479:c145633-145572 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin