Detailed information of TA system TA216510

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 107228..107497
• Replicon: plasmid unnamed1
• Accession: NZ_CP083479
• Organism: Escherichia coli strain elppa10

Toxin (Protein)

• Gene name: hok
• Uniprot ID: G9G195
• Locus tag: K9U13_RS23755
• Protein ID: WP_001323520.1
• Coordinates: 107381..107497 (+)
• Length: 39 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	107228..107293 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9U13_RS23700	102447..103418	+	972	WP_000817028.1	ParB/RepB/Spo0J family plasmid partition protein	-
K9U13_RS23705	104055..104224	+	170	Protein_105	hypothetical protein	-
K9U13_RS23710	104407..104487	-	81	Protein_106	hypothetical protein	-
K9U13_RS23715	104557..104763	+	207	WP_000275856.1	hypothetical protein	-
K9U13_RS23720	104789..105328	+	540	WP_000290840.1	single-stranded DNA-binding protein	-
K9U13_RS23725	105396..105629	+	234	WP_000005987.1	DUF905 family protein	-
K9U13_RS23730	105657..105854	+	198	Protein_110	hypothetical protein	-
K9U13_RS23735	105909..106343	+	435	WP_000845936.1	conjugation system SOS inhibitor PsiB	-
K9U13_RS23740	106340..107102	+	763	Protein_112	plasmid SOS inhibition protein A	-
K9U13_RS23745	107071..107259	-	189	WP_001299721.1	hypothetical protein	-
-	107071..107295	+	225	NuclAT_0	-	-
-	107071..107295	+	225	NuclAT_0	-	-
-	107071..107295	+	225	NuclAT_0	-	-
-	107071..107295	+	225	NuclAT_0	-	-
-	107228..107293	-	66	-	-	Antitoxin
K9U13_RS23750	107281..107430	+	150	Protein_114	plasmid maintenance protein Mok	-
K9U13_RS23755	107381..107497	+	117	WP_001323520.1	type I toxin-antitoxin system Hok family toxin	Toxin
K9U13_RS23760	107717..107947	+	231	WP_071586998.1	hypothetical protein	-
K9U13_RS23765	107945..108117	-	173	Protein_117	hypothetical protein	-
K9U13_RS23770	108187..108393	+	207	WP_000547968.1	hypothetical protein	-
K9U13_RS23775	108418..108705	+	288	WP_000107535.1	hypothetical protein	-
K9U13_RS23780	108823..109644	+	822	WP_001234426.1	DUF932 domain-containing protein	-
K9U13_RS23785	109941..110543	-	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
K9U13_RS23790	110864..111247	+	384	WP_001151524.1	conjugal transfer relaxosome DNA-binding protein TraM	-
K9U13_RS23795	111434..112123	+	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(3)-IId / blaTEM-1A / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / floR / dfrA14 / sul3 / aph(3')-Ia / sitABCD	iroE / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA	1..149801	149801

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (1-34)

Sequences

Toxin

Protein

• Length: 39 a.a.
• Molecular weight: 4455.23 Da
• Isoelectric Point: 8.5110

>T216510 WP_001323520.1 NZ_CP083479:107381-107497 [Escherichia coli]
VCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 117 bp

>T216510 NZ_CP113096:c3369006-3368593 [Pseudomonas citronellolis]
ATGCTGAAGTTCATGCTCGATACCAACATCTGCATCTTCACCATCAAGAACAAGCCGCAAGTCGTTCGCGAGGCCTTCAA
CCAGCACCATGGGCAGTTGGCGATCAGCTCGGTGACGCTGATGGAACTGATATACGGCGCCGAGAAGTCCTCCATGCCGG
AACACAACCTGGGCATCGTCGAAGGCTTCGCCGCCCGCCTGGAAGTGCTCGACTACGACGGCAACGCGGCGGCCCACACC
GGGCAACTGCGTGCCGAGCTGGCGCGAATCGGCAAGCCCATCGGCCCCTACGACCAGATGATCGCCGGGCACGCCCGTTC
CCGCGGGCTGATTCTGGTCAGCAACAACCTGCGCGAATTCGAGCAAGTGCCGGGGTTGCGCCTGGAGGACTGGACCGTAG
CCCCATCGCCTTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT216510 NZ_CP083479:c107293-107228 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Toxin

• AlphaFold DB: G9G195

Antitoxin