Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4783565..4784106 Replicon chromosome
Accession NZ_CP083478
Organism Escherichia coli strain elppa10

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag K9U13_RS23080 Protein ID WP_000615976.1
Coordinates 4783828..4784106 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag K9U13_RS23075 Protein ID WP_000729704.1
Coordinates 4783565..4783825 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9U13_RS23050 (4779348) 4779348..4780133 - 786 WP_000207575.1 putative lateral flagellar export/assembly protein LafU -
K9U13_RS23055 (4780105) 4780105..4781817 + 1713 Protein_4489 flagellar biosynthesis protein FlhA -
K9U13_RS23060 (4781922) 4781922..4782200 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
K9U13_RS23065 (4782193) 4782193..4782549 + 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin -
K9U13_RS23070 (4782606) 4782606..4783379 - 774 WP_000543895.1 C40 family peptidase -
K9U13_RS23075 (4783565) 4783565..4783825 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
K9U13_RS23080 (4783828) 4783828..4784106 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
K9U13_RS23085 (4784262) 4784262..4785002 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
K9U13_RS23090 (4784973) 4784973..4785740 - 768 WP_000333380.1 class II glutamine amidotransferase -
K9U13_RS23095 (4785946) 4785946..4786524 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA / rhs/PAAR 4782193..4800092 17899


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T216508 WP_000615976.1 NZ_CP083478:4783828-4784106 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T216508 NZ_CP113096:2940319-2940570 [Pseudomonas citronellolis]
ATGTCCAGGAACGAGAAACTGCTGGCCAAGCTACTCAACGAAAGCGCCAGCTTTACCTGGCAGGAGCTTGTGACACTGCT
GCGTTGGCTGGGCTACAGGCAGTTGGAGGGCAGCGGGAGCCGGGTCAAGTTCGACAACGGCGACCCGCAGGCGTTGATCA
ACCTTCACAAGCCGCACCCGTGCAACGAGCTGAAAGCCTATGTCCGGCGCCAGATCATCGAGCACCTCAAGGCGGGGGAA
CTGATTCCGTGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT216508 WP_000729704.1 NZ_CP083478:4783565-4783825 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT216508 NZ_CP113096:2940567-2940908 [Pseudomonas citronellolis]
GTGAACAACCTGCTGCAATACCGGGGCTATTACGGCTCCATCGAAGCCAGCACCGAGGACAACTGCCTGTTCGGCAAGCT
GCTCTTCATCCGCGCGCTGGTCAACTACGAAGGCGAGACGGTGGCCGAACTGCAGGCCGCTTTCCGCGAGGCGGTGGACG
ACTACCTGGCCACATGCCAGGCGCAGGGGCAGGAGCCGGAAGTGCCTTGCAAGGGATCGTTCAACGTGCGGGTCGGCCAT
GACTTGCATCTGGCAGCGAGCCTGGCAGCCAACCGGCAGCACATGAGCCTGAACGACCTCACCCGGCAGGCGCTGAGCGA
GTATTTGCAGCACCACGGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References