Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 1631220..1632019 Replicon chromosome
Accession NZ_CP083478
Organism Escherichia coli strain elppa10

Toxin (Protein)


Gene name yhaV Uniprot ID F4VJD3
Locus tag K9U13_RS07715 Protein ID WP_000347266.1
Coordinates 1631220..1631684 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag K9U13_RS07720 Protein ID WP_001307405.1
Coordinates 1631684..1632019 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9U13_RS07685 (1626221) 1626221..1626655 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
K9U13_RS07690 (1626673) 1626673..1627551 - 879 WP_001315856.1 PTS N-acetylgalactosamine transporter subunit IID -
K9U13_RS07695 (1627541) 1627541..1628320 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
K9U13_RS07700 (1628331) 1628331..1628804 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
K9U13_RS07705 (1628827) 1628827..1630107 - 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
K9U13_RS07710 (1630356) 1630356..1631165 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
K9U13_RS07715 (1631220) 1631220..1631684 - 465 WP_000347266.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
K9U13_RS07720 (1631684) 1631684..1632019 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
K9U13_RS07725 (1632168) 1632168..1633739 - 1572 WP_001273741.1 galactarate dehydratase -
K9U13_RS07730 (1634114) 1634114..1635448 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
K9U13_RS07735 (1635464) 1635464..1636234 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17841.18 Da        Isoelectric Point: 9.4947

>T216489 WP_000347266.1 NZ_CP083478:c1631684-1631220 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRFFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T216489 NZ_CP113085:c96168-95989 [Pantoea agglomerans]
ATGGATAGCAGCACGTTAATTTCCGAAATGAAGGCTGATGGCTGGGTTCTGACAGGGATAAACGGTAGCCATCACCATTT
CACACATCCGACAAAACCGGGGCTGGTTACAGTACCCCATCCTAAAAAAGACTTACCGATCGGTACGGTAAAGAGCATCA
GGAAACAAGCGAGAATTTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT216489 WP_001307405.1 NZ_CP083478:c1632019-1631684 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT216489 NZ_CP113085:c95962-95537 [Pantoea agglomerans]
ATGTTTTATCCAATCGCGATTGAAGCGGGTGATCACGAACACGCATACGGCGTAATCGTTCCCGATTTACCAGGGTGTTT
CTCTGCCGGGGATACTCTTGATGAAGCCATTAAAAACGCCAAAGAGGCTATCACAGGGCATATCGAATTATGTGTAGAGC
TCGGTCATGAGATCCCTGCCGTGTCCACCATTGAGACTCTGGCCACGAATCCTGACTATCAGGGTTATATCTGGGCATTG
GTTGATGTAGATGTCATTCGCTTGCTGGGCGGCTCTGAAAAAATCAATGTCACTTTACCCAGGTCGTTAATTGATCGGAT
TGATCGTTGTGTGGCTTCACACCCCGAATACAAAAGTCGCTCGGGCTTTCTGGCTCAGGTAGCGCTTGAGCGTATTACTG
CTCCGATTAACAGGCCTCAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A836NGD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References