Detailed information of TA system TA216486

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 818384..818986
• Replicon: chromosome
• Accession: NZ_CP083478
• Organism: Escherichia coli strain elppa10

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: K9U13_RS03750
• Protein ID: WP_000897305.1
• Coordinates: 818675..818986 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K9U13_RS03745
• Protein ID: WP_000356397.1
• Coordinates: 818384..818674 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9U13_RS03720 (813764)	813764..814666	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
K9U13_RS03725 (814663)	814663..815298	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
K9U13_RS03730 (815295)	815295..816224	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
K9U13_RS03735 (816554)	816554..816796	-	243	WP_001087409.1	protein YiiF	-
K9U13_RS03745 (818384)	818384..818674	-	291	WP_000356397.1	NadS family protein	Antitoxin
K9U13_RS03750 (818675)	818675..818986	-	312	WP_000897305.1	hypothetical protein	Toxin
K9U13_RS03755 (819215)	819215..820123	+	909	WP_001162704.1	alpha/beta hydrolase	-
K9U13_RS03760 (820187)	820187..821128	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
K9U13_RS03765 (821173)	821173..821610	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
K9U13_RS03770 (821607)	821607..822479	-	873	WP_000920762.1	virulence factor BrkB family protein	-
K9U13_RS03775 (822473)	822473..823072	-	600	WP_001295269.1	glucose-1-phosphatase	-
K9U13_RS03780 (823171)	823171..823956	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T216486 WP_000897305.1 NZ_CP083478:c818986-818675 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T216486 NZ_CP113083:c3933149-3932745 [Rhodopseudomonas palustris]
GTGAGGGCGTTCTTCGATACCAATATTTTGGTCTACTCCACGACGTCGGACCCGCGGCAAGCAACCGCCGCCGCTTGTTT
GGAGCAGGGCGGGTTTGCCAGCGTTCAGGTGCTCAACGAATTCGTCCACGTGGCGCGCCGCAAGCTGCGGCACGACTGGC
CGCAGATCGAAGTCGCGCTCGAGCAGTTTCACGCCGCGCTCGATGACGTACTTCCGATCACTCTAACCACGCATGCCGCA
GCTGTTGTCCTTGCACGCGATCACCGGGTGTCGTTCTACGACGCGCTGATCGTCGCGGCGGCCCAAGCGGCTGGCTGCGA
TGTGCTGTACAGCGAAGACCTGCAGCACGGCCGCACCTTCGGCGCGCTGCGCGTCGAGAATCCATTTCTGGAAGGCTCGC
GATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT216486 WP_000356397.1 NZ_CP083478:c818674-818384 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT216486 NZ_CP113083:c3933400-3933146 [Rhodopseudomonas palustris]
ATGCTGGTTTCGAAATGGGGCAACAGTCTCGCCGTCAGGCTGCCCAAGGCGTTGGTCGACAAGCTCGATCTGAAAGAGGG
CGACGAGTTGAACGTCGTCGACATCGTCGAGCGGACGCTGGTGGTCGAGAAGGAGGACAGGCGGAAAGCAGCGGTGGAGC
GGCTTTCCAGGCTGCGATGGACCCTTCCTACCGACTACAAATTCGATCGCGACGAGGCGAACGAGCGCTATCGCGACGAG
TCGAACGAGCCGTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7