Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2980733..2981379 Replicon chromosome
Accession NZ_CP083445
Organism Klebsiella pneumoniae strain KP294

Toxin (Protein)


Gene name higB Uniprot ID A0A483JFR4
Locus tag LAV94_RS14465 Protein ID WP_004188313.1
Coordinates 2980733..2981080 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A483GM64
Locus tag LAV94_RS14470 Protein ID WP_004188315.1
Coordinates 2981080..2981379 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LAV94_RS14455 (2976659) 2976659..2978092 + 1434 WP_002920564.1 glycogen synthase GlgA -
LAV94_RS14460 (2978110) 2978110..2980557 + 2448 WP_002920561.1 glycogen phosphorylase -
LAV94_RS14465 (2980733) 2980733..2981080 + 348 WP_004188313.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LAV94_RS14470 (2981080) 2981080..2981379 + 300 WP_004188315.1 XRE family transcriptional regulator Antitoxin
LAV94_RS14475 (2981442) 2981442..2982950 - 1509 WP_002920554.1 glycerol-3-phosphate dehydrogenase -
LAV94_RS14480 (2983155) 2983155..2983484 + 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
LAV94_RS14485 (2983535) 2983535..2984365 + 831 WP_004188317.1 rhomboid family intramembrane serine protease GlpG -
LAV94_RS14490 (2984415) 2984415..2985173 + 759 WP_002920548.1 DeoR/GlpR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-78)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13548.57 Da        Isoelectric Point: 6.2327

>T216454 WP_004188313.1 NZ_CP083445:2980733-2981080 [Klebsiella pneumoniae]
MWDVETTDTFDTWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGNKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T216454 NZ_CP113051:c1685285-1685034 [Staphylococcus aureus]
ATGAATAACCGCGAACAAATTGAACAATCAATTATCAGTGCTAGTGCCTATAACGGTAATGACACAGAGGGATTACTAAA
AGAGGTTGAAGACGTGTATAAGAAAGCGCAAGCGTTTGATGAAATACTTGATGGAATGACAAATGCTATTCAACATTCAG
TTAAAGAAGGTGTTGAACTTGATGAAGCAGTAGGGATTATGGCAGGTCAAGTTGTCTATAAATATGAGGAGGAACAGGAA
AATGAGTATTAG

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10873.64 Da        Isoelectric Point: 10.1464

>AT216454 WP_004188315.1 NZ_CP083445:2981080-2981379 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAAWG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT216454 NZ_CP113051:c1685044-1684871 [Staphylococcus aureus]
ATGAGTATTAGTGTAGGAGATAAAGTATATAACCATGAAACAAACGAAAGTCTAGAGATTGTGCAATTGGTCGGAGATAT
TAGAGATACACATTATAAACTGTCCGATGATTCAGTTATTAGCATTATAGATTTTATTACTAAACCAATTTATCTAATTA
AGGGGGACGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A483JFR4

Antitoxin

Source ID Structure
AlphaFold DB A0A483GM64

References