Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 311862..312556 Replicon chromosome
Accession NC_008258
Organism Shigella flexneri 5 str. 8401

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag SFV_RS01655 Protein ID WP_001263491.1
Coordinates 311862..312260 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID Q0T7Q4
Locus tag SFV_RS01660 Protein ID WP_000554759.1
Coordinates 312263..312556 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SFV_RS01630 307201..307659 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
SFV_RS01635 307921..309378 + 1458 WP_001293029.1 cytosol nonspecific dipeptidase -
SFV_RS01640 309435..310049 - 615 Protein_308 peptide chain release factor H -
SFV_RS01645 310111..311154 - 1044 WP_005060486.1 RNA ligase RtcB family protein -
SFV_RS01650 311400..311852 - 453 WP_001059862.1 GNAT family N-acetyltransferase -
SFV_RS01655 311862..312260 - 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
SFV_RS01660 312263..312556 - 294 WP_000554759.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
SFV_RS01665 312608..313663 - 1056 WP_001226172.1 DNA polymerase IV -
SFV_RS01670 313734..314519 - 786 WP_000207560.1 putative lateral flagellar export/assembly protein LafU -
SFV_RS01675 314491..316203 + 1713 Protein_315 flagellar biosynthesis protein FlhA -
SFV_RS01680 316420..316917 - 498 WP_000006250.1 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - gtrB 220475..317647 97172


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T21645 WP_001263491.1 NC_008258:c312260-311862 [Shigella flexneri 5 str. 8401]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T21645 NC_008258:c312260-311862 [Shigella flexneri 5 str. 8401]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11232.82 Da        Isoelectric Point: 7.0153

>AT21645 WP_000554759.1 NC_008258:c312556-312263 [Shigella flexneri 5 str. 8401]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
KQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT21645 NC_008258:c312556-312263 [Shigella flexneri 5 str. 8401]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
AGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTTCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
AAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TR46

References