Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 4052538..4053135 Replicon chromosome
Accession NZ_CP083443
Organism Klebsiella pneumoniae strain KP1711R

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag K9870_RS19730 Protein ID WP_004142563.1
Coordinates 4052818..4053135 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag K9870_RS19725 Protein ID WP_004142561.1
Coordinates 4052538..4052825 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9870_RS19695 (4048519) 4048519..4048767 + 249 WP_020325243.1 DUF1158 domain-containing protein -
K9870_RS19700 (4048785) 4048785..4049126 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
K9870_RS19705 (4049157) 4049157..4050272 - 1116 WP_020325248.1 MBL fold metallo-hydrolase -
K9870_RS19710 (4050452) 4050452..4051132 + 681 WP_119062547.1 TetR/AcrR family transcriptional regulator -
K9870_RS19715 (4051132) 4051132..4051500 + 369 WP_020325252.1 MmcQ/YjbR family DNA-binding protein -
K9870_RS19720 (4051620) 4051620..4052273 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
K9870_RS19725 (4052538) 4052538..4052825 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
K9870_RS19730 (4052818) 4052818..4053135 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
K9870_RS19735 (4053320) 4053320..4054363 - 1044 WP_020325241.1 DUF2157 domain-containing protein -
K9870_RS19740 (4055033) 4055033..4055899 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
K9870_RS19745 (4056008) 4056008..4057435 + 1428 WP_009308097.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T216438 WP_004142563.1 NZ_CP083443:c4053135-4052818 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T216438 NZ_CP113047:c1904785-1904597 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT216438 WP_004142561.1 NZ_CP083443:c4052825-4052538 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT216438 NZ_CP113047:c1905074-1904814 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATCGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTATATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References