Detailed information of TA system TA216405

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 2244192..2244709
• Replicon: chromosome
• Accession: NZ_CP083434
• Organism: Staphylococcus aureus strain DC.RB_015

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: A0A2S6D1N9
• Locus tag: LAE57_RS10685
• Protein ID: WP_000113263.1
• Coordinates: 2244443..2244709 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: T1YB60
• Locus tag: LAE57_RS10680
• Protein ID: WP_000584499.1
• Coordinates: 2244192..2244443 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LAE57_RS10650 (2239892)	2239892..2240611	+	720	WP_000479576.1	amino acid ABC transporter permease	-
LAE57_RS10655 (2240608)	2240608..2241339	+	732	WP_000615464.1	amino acid ABC transporter ATP-binding protein	-
LAE57_RS10660 (2241436)	2241436..2242686	-	1251	WP_001012236.1	FemA/FemB family glycyltransferase FmhA	-
LAE57_RS10665 (2242941)	2242941..2243303	+	363	WP_000819839.1	DUF4467 domain-containing protein	-
LAE57_RS10670 (2243322)	2243322..2243921	+	600	WP_000162810.1	DsbA family protein	-
LAE57_RS10675 (2243928)	2243928..2244019	+	92	Protein_2109	hypothetical protein	-
LAE57_RS10680 (2244192)	2244192..2244443	+	252	WP_000584499.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LAE57_RS10685 (2244443)	2244443..2244709	+	267	WP_000113263.1	Txe/YoeB family addiction module toxin	Toxin
LAE57_RS10690 (2244744)	2244744..2244815	+	72	WP_223200558.1	hypothetical protein	-
LAE57_RS10695 (2245003)	2245003..2246550	+	1548	WP_000727757.1	zinc ABC transporter substrate-binding lipoprotein AdcA	-
LAE57_RS10700 (2246736)	2246736..2247203	+	468	WP_000153338.1	SRPBCC domain-containing protein	-
LAE57_RS10705 (2247290)	2247290..2247472	+	183	WP_000079140.1	hypothetical protein	-
LAE57_RS10710 (2247676)	2247676..2248500	+	825	WP_000594633.1	formate/nitrite transporter family protein	-
LAE57_RS10715 (2248913)	2248913..2249449	+	537	WP_000185901.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (8-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10437.01 Da
• Isoelectric Point: 10.1628

>T216405 WP_000113263.1 NZ_CP083434:2244443-2244709 [Staphylococcus aureus]
MARLNITFSPQAFEDYKYFQQNDKKMVKKINELLKSIDRNGALKGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Nucleotide

• Length: 267 bp

>T216405 NZ_CP113039:c839407-839141 [Staphylococcus aureus]
ATGAGCAATTACACGGTTAAGATTAAAAATTCAGCGAAATCAGATTTAAGGAAAATAAAACATTCTTATTTAAAGAAGTC
ATTTTTAGAAATTGTTGAGACTTTAAAAAATGATCCGTATAAAATAACACAATCTTTTGAAAAATTAGAGCCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCATCAGCACAGGGTCGTCTATACCGTAGATGATCGAAATAAAGAAGTATTAATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9433.60 Da
• Isoelectric Point: 4.3407

>AT216405 WP_000584499.1 NZ_CP083434:2244192-2244443 [Staphylococcus aureus]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Nucleotide

• Length: 252 bp

>AT216405 NZ_CP113039:c839664-839407 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGCAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATAT
ATCTTGAATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAATAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTTTAA

Structures

Toxin

• AlphaFold DB: A0A2S6D1N9

Antitoxin

• AlphaFold DB: T1YB60