Detailed information of TA system TA021636

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4281944..4282546
• Replicon: chromosome
• Accession: NC_008253
• Organism: Escherichia coli 536

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: ECP_RS20860
• Protein ID: WP_000897305.1
• Coordinates: 4281944..4282255 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ECP_RS20865
• Protein ID: WP_000356397.1
• Coordinates: 4282256..4282546 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECP_RS20835	4277858..4278457	+	600	WP_001298594.1	glucose-1-phosphatase	-
ECP_RS20840	4278451..4279323	+	873	WP_000920762.1	virulence factor BrkB family protein	-
ECP_RS20845	4279320..4279757	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
ECP_RS20850	4279802..4280743	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
ECP_RS20855	4280807..4281715	-	909	WP_001298596.1	alpha/beta hydrolase	-
ECP_RS20860	4281944..4282255	+	312	WP_000897305.1	hypothetical protein	Toxin
ECP_RS20865	4282256..4282546	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
ECP_RS20875	4283151..4283369	+	219	WP_001298592.1	ribbon-helix-helix domain-containing protein	-
ECP_RS20880	4283584..4284513	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
ECP_RS20885	4284510..4285145	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
ECP_RS20890	4285142..4286044	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12172.16 Da
• Isoelectric Point: 9.7791

>T21636 WP_000897305.1 NC_008253:4281944-4282255 [Escherichia coli 536]
ILFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T21636 NC_008253:4281944-4282255 [Escherichia coli 536]
ATCCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT21636 WP_000356397.1 NC_008253:4282256-4282546 [Escherichia coli 536]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT21636 NC_008253:4282256-4282546 [Escherichia coli 536]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7