Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 141716..142327 Replicon plasmid p1
Accession NZ_CP083348
Organism Ensifer adhaerens strain W2-B

Toxin (Protein)


Gene name graT Uniprot ID -
Locus tag LAC80_RS35300 Protein ID WP_223730852.1
Coordinates 142046..142327 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag LAC80_RS35295 Protein ID WP_223730851.1
Coordinates 141716..142015 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LAC80_RS35270 (LAC80_35055) 138119..138952 + 834 WP_223730849.1 pyrroline-5-carboxylate reductase -
LAC80_RS35275 (LAC80_35060) 139030..139224 + 195 WP_223730850.1 tautomerase family protein -
LAC80_RS35280 (LAC80_35065) 140566..140927 + 362 Protein_136 DUF4112 domain-containing protein -
LAC80_RS35285 141111..141191 - 81 WP_223731031.1 type II toxin-antitoxin system MqsA family antitoxin -
LAC80_RS35290 141532..141696 - 165 WP_223731086.1 hypothetical protein -
LAC80_RS35295 (LAC80_35080) 141716..142015 - 300 WP_223730851.1 HigA family addiction module antitoxin Antitoxin
LAC80_RS35300 (LAC80_35085) 142046..142327 - 282 WP_223730852.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LAC80_RS35310 (LAC80_35095) 142928..143488 - 561 WP_223730854.1 hypothetical protein -
LAC80_RS35315 (LAC80_35100) 144336..144811 + 476 Protein_142 DUF892 family protein -
LAC80_RS35320 (LAC80_35105) 144862..145521 + 660 WP_223730855.1 hypothetical protein -
LAC80_RS35325 (LAC80_35110) 146579..146797 - 219 Protein_144 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - htpB 1..735984 735984


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(2-93)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(6-80)


Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10412.83 Da        Isoelectric Point: 9.1204

>T216296 WP_223730852.1 NZ_CP083348:c142327-142046 [Ensifer adhaerens]
VIRSFKDAKTEAVARGKAPKGFPSDIVRSAVRKLTILNNAAALEDLRSPPGNRLEALKGNREGQHSIRINDQWRICFVWT
DAGIEDVEIVDYH

Download         Length: 282 bp

>T216296 NZ_CP113007:682436-682702 [Staphylococcus aureus]
ATGAGCAATTACACGGTTAAGATTAAAAATTCAGCGAAATCAGATTTAAAGAAAATAAAACATTCTTATTTAAAGAAGTC
ATTTTTAGAAATTGTTGAGACTTTAAAAAATGATCCGTATAAAATAACACAATCTTTTGAAAAATTAGAGCCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCATCAGCACAGGGTCGTCTATACCGTAGATGATCGAAATAAAGAAGTATTAATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 11312.13 Da        Isoelectric Point: 6.2263

>AT216296 WP_223730851.1 NZ_CP083348:c142015-141716 [Ensifer adhaerens]
MTHILPPVHPGEILREEYLLPLNMSAGALAKKLNVPRTRIERIAAEQTPITTDTALRLAKYFRTTPEFWMNMQTSFNLKT
EEVALHSELELIPEIDRAA

Download         Length: 300 bp

>AT216296 NZ_CP113007:682179-682436 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGCAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATAT
ATCTTGAATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAATAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References